Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Rev Med Virol. 2019 Jan;29(1):e2009. doi: 10.1002/rmv.2009. Epub 2018 Sep 27.
The recent development of the Clustered Regularly Interspaced Palindromic Repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, a genome editing system, has many potential applications in virology. The possibility of introducing site specific breaks has provided new possibilities to precisely manipulate viral genomics. Here, we provide diagrams to summarize the steps involved in the process. We also systematically review recent applications of the CRISPR/Cas9 system for manipulation of DNA virus genomics and discuss the therapeutic potential of the system to treat viral diseases.
最近，CRISPR/CRISPR 相关蛋白 9 (Cas9) 基因组编辑系统的发展在病毒学中有许多潜在的应用。该系统能够在特定位置产生断裂，为精确操作病毒基因组提供了新的可能。在这里，我们提供了图表来总结这一过程所涉及的步骤。我们还系统地回顾了 CRISPR/Cas9 系统在 DNA 病毒基因组操作中的最新应用，并讨论了该系统治疗病毒病的治疗潜力。
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