Kuo C F, Fridovich I
Biochem J. 1986 Jul 15;237(2):505-10. doi: 10.1042/bj2370505.
The superoxide radical O2.-, whether produced by the xanthine/xanthine oxidase reaction or infused as KO2, solubilized by a crown ether in dry dimethyl sulphoxide, initiated a free-radical chain oxidation of anionic 2-nitropropane. Superoxide dismutase, but not catalase, inhibited oxidation of the nitroalkane. Xanthine oxidase suffered a syncatalytic inactivation, during the co-oxidation of 2-nitropropane, which was reversed by dialysis. Cyanide exacerbated this syncatalytic inactivation and rendered it irreversible. The frequently observed oxidations of nitroalkanes by flavoenzymes now need to be re-examined to clarify the extent to which O2.--initiated free-radical chain oxidation contributed to the overall nitroalkane oxidation.
超氧阴离子自由基O2.-，无论是通过黄嘌呤/黄嘌呤氧化酶反应产生，还是作为超氧化钾注入，并通过冠醚溶解于干燥的二甲基亚砜中，都引发了阴离子2-硝基丙烷的自由基链式氧化反应。超氧化物歧化酶可抑制硝基烷的氧化，而过氧化氢酶则不能。在2-硝基丙烷的共氧化过程中，黄嘌呤氧化酶发生了同步催化失活，透析可使其恢复。氰化物加剧了这种同步催化失活并使其不可逆。目前需要重新审视黄素酶对硝基烷的常见氧化作用，以阐明O2.-引发的自由基链式氧化在硝基烷整体氧化中所起作用的程度。
