Khamkeaw Arnon, Phisalaphong Muenduen
Chemical Engineering Research Unit for Value Adding of Bioresources, Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, 10330 Thailand.
Food Sci Biotechnol. 2016 Apr 30;25(2):509-516. doi: 10.1007/s10068-016-0071-1. eCollection 2016.
Loog-Pang (Thai rice cake starter) is an effective and inexpensive microbial source for the hydrolysis of cassava starch to glucose. A process for hydrolysis of cassava starch to glucose by Loog- Pang was improved by co-immobilized multi-microorganisms (IC) using thin shell silk cocoon (TSC). After incubation at 35°C for 120 h, the IC-TSC system converted 20% w/v cassava starch slurry into clear glucose syrup containing a glucose concentration of 145.5 g/L (composed of 98.8% glucose and 1.2% oligosaccharides), with little or no contamination by microorganisms. The glucose concentration from the starch hydrolysis process using the IC-TSC system was approximately 1.3 times more than that of suspended cultures (SC). The starch hydrolysate could be used as the carbon source for ethanol fermentation without sterilization. A concentration of ethanol of 71.2 g/L (9.1%, v/v) was obtained at 36 h fermentation of the starch hydrolysate by M30.
鲁格-庞（泰国米糕发酵剂）是一种将木薯淀粉水解为葡萄糖的有效且廉价的微生物来源。通过使用薄壳蚕茧（TSC）共固定化多种微生物（IC），改进了鲁格-庞将木薯淀粉水解为葡萄糖的工艺。在35°C下孵育120小时后，IC-TSC系统将20% w/v的木薯淀粉浆转化为清澈的葡萄糖糖浆，其中葡萄糖浓度为145.5 g/L（由98.8%的葡萄糖和1.2%的寡糖组成），微生物污染很少或没有污染。使用IC-TSC系统进行淀粉水解过程得到的葡萄糖浓度约为悬浮培养（SC）的1.3倍。淀粉水解产物无需灭菌即可用作乙醇发酵的碳源。在M30对淀粉水解产物进行36小时发酵后，获得了浓度为71.2 g/L（9.1%，v/v）的乙醇。
