一种用于快速检测含益生菌片剂中孢子的简单流式细胞术方法的应用。
The use of a simple flow cytometry method for rapid detection of spores in probiotic -containing tablets.
作者信息
Zheng Xiao-Ling, Xiong Zhi-Qiang, Wu Jie-Qun
机构信息
1Zhejiang Institute for Food and Drug Control, Hangzhou, 310004 China.
2School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093 China.
出版信息
Food Sci Biotechnol. 2017 Feb 28;26(1):167-171. doi: 10.1007/s10068-017-0022-5. eCollection 2017.
Detection of the number of vegetative cells and endospores is necessary for quality control during the production of orally administered probiotic -containing tablets (BCT). However, there is no standard method for the rapid detection of vegetative cells and endospores in China. In this study, a simple flow cytometry (FCM) method was used to monitor the population dynamics of BCT. Using a specific fluorescent stain, SYBR green I, flow cytometric analysis could easily differentiate two morphological states of . Compared with plate count assay (PCA) for determining the number of vegetative cells and endospores, the percentage of endospores determined by FCM was ~10% higher than that by PCA. Advantages of the FCM method over conventional methods include lower labor work, shorter detection time, and higher accuracy. Therefore, this simple FCM method could be a practical tool for monitoring quality control during the production of probiotic BCT.
在含益生菌口服片剂（BCT）的生产过程中，检测营养细胞和芽孢的数量对于质量控制而言是必要的。然而，在中国尚无快速检测营养细胞和芽孢的标准方法。在本研究中，采用了一种简单的流式细胞术（FCM）方法来监测BCT的群体动态。使用特异性荧光染料SYBR green I，流式细胞术分析能够轻松区分两种形态状态。与用于确定营养细胞和芽孢数量的平板计数法（PCA）相比，通过FCM测定的芽孢百分比比PCA高约10%。FCM方法相对于传统方法的优势包括较低的劳动强度、更短的检测时间以及更高的准确性。因此，这种简单的FCM方法可能是监测益生菌BCT生产过程中质量控制的实用工具。
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