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地衣芽孢杆菌固态发酵高产孢子生产

High-yield spore production from Bacillus licheniformis by solid state fermentation.

作者信息

Zhao Shumiao, Hu Nan, Huang Jun, Liang Yunxiang, Zhao Bin

机构信息

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China.

出版信息

Biotechnol Lett. 2008 Feb;30(2):295-7. doi: 10.1007/s10529-007-9540-1. Epub 2007 Sep 18.

Abstract

Bacillus licheniformis was grown for 48 h at 37 degrees C in solid state fermentation; a maximum of 1.7 x 10(11) spores/g dry substrate were obtained using rice straw powder (300 g/kg) and wheat bran (700 g/kg) supplemented with glucose (40 g/kg), peptone (20 g/kg), yeast extract (20 g/kg), KH(2)PO(4) (10 g/kg) and CaO (5 g/kg) with an initial moisture content of 65%.

摘要

地衣芽孢杆菌在37℃下进行固态发酵培养48小时;使用添加了葡萄糖(40克/千克)、蛋白胨(20克/千克)、酵母提取物(20克/千克)、磷酸二氢钾(10克/千克)和氧化钙(5克/千克)的稻草粉(300克/千克)和麦麸(700克/千克),初始水分含量为65%,可获得最高1.7×10¹¹个孢子/克干基质。

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