Zhao Shumiao, Hu Nan, Huang Jun, Liang Yunxiang, Zhao Bin
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China.
Biotechnol Lett. 2008 Feb;30(2):295-7. doi: 10.1007/s10529-007-9540-1. Epub 2007 Sep 18.
Bacillus licheniformis was grown for 48 h at 37 degrees C in solid state fermentation; a maximum of 1.7 x 10(11) spores/g dry substrate were obtained using rice straw powder (300 g/kg) and wheat bran (700 g/kg) supplemented with glucose (40 g/kg), peptone (20 g/kg), yeast extract (20 g/kg), KH(2)PO(4) (10 g/kg) and CaO (5 g/kg) with an initial moisture content of 65%.
地衣芽孢杆菌在37℃下进行固态发酵培养48小时;使用添加了葡萄糖(40克/千克)、蛋白胨(20克/千克)、酵母提取物(20克/千克)、磷酸二氢钾(10克/千克)和氧化钙(5克/千克)的稻草粉(300克/千克)和麦麸(700克/千克),初始水分含量为65%,可获得最高1.7×10¹¹个孢子/克干基质。