Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province, Zhejiang A & F University, Hangzhou, 311300, China.
Zhejiang Provincial Collaborative Innovation Center for Bamboo Resources and High-efficiency Utilization, Hangzhou, 311300, China.
Environ Sci Pollut Res Int. 2018 Nov;25(33):33432-33442. doi: 10.1007/s11356-018-3271-9. Epub 2018 Sep 28.
Biochar amendment has been advocated as an effective method to remediate organic pollutant-contaminated soils through adsorption and stimulating microbial degradation. However, such effects can vary depending on soil properties and biochar physiochemical characteristics. The objective of this study was to compare the dynamic variations of polychlorinated biphenyls (PCBs) in low and high soil organic carbon (SOC) soils both amended with biochar and to investigate its linkage with fungal community composition. Two soils having the same texture and soil type but varying in SOC contents were contaminated with PCBs to a final concentration of 60 mg kg and amended with 2% bamboo biochar. Temporal changes of PCB remaining in soils and adsorbed on biochar particles were determined during a 2-month incubation. Diversity and composition of fungal communities in both low and high SOC soils were investigated with Illumina MiSeq sequencing. The results showed that the PCB concentrations in low SOC soil were significantly lower than those in high SOC soil during the incubation. In the low SOC soil, the biochar particle adsorbed higher amounts of PCB, tetra-, and penta-chlorobiphenyls (CBs) than those in high SOC soil, and stimulated the dissipation of di- and tri-CBs. The nonmetric multidimensional scaling profile showed significant (p < 0.05) differences in the fungal community composition between the low and high SOC soils. The relative abundances of Eurotiomycetes were gradually increased, whereas those of Sordariomycetes and Dothideomycetes were decreased with increasing incubation time in the low SOC soils. In contrast, the fungal communities in high SOC soils were relatively stable. The relative abundances of Eurotiomycetes and Sordariomycetes were positively correlated with PCB dissipation in low and high SOC content soils, respectively. Our results suggest that SOC content affects PCB dissipation and fungal community composition in biochar-amended soils, and biochars have a high remediation potential of PCB in soils with low SOC contents.
生物炭的添加被认为是一种通过吸附和刺激微生物降解来修复有机污染物污染土壤的有效方法。然而，这种效果可能因土壤性质和生物炭物理化学特性而异。本研究的目的是比较添加生物炭后低有机碳（SOC）和高 SOC 土壤中多氯联苯（PCBs）的动态变化，并研究其与真菌群落组成的关系。选择两种质地和土壤类型相同但 SOC 含量不同的土壤，将其污染至最终浓度为 60mg/kg，并添加 2%的竹炭。在 2 个月的培养过程中，测定了土壤中 PCB 残留量和吸附在生物炭颗粒上的 PCB 量的时间变化。利用 Illumina MiSeq 测序技术研究了低 SOC 和高 SOC 土壤中真菌群落的多样性和组成。结果表明，在培养过程中，低 SOC 土壤中的 PCB 浓度明显低于高 SOC 土壤。在低 SOC 土壤中，生物炭颗粒吸附的四氯和五氯联苯（CBs）比高 SOC 土壤多，并且刺激了二氯和三氯联苯的降解。非度量多维尺度分析谱显示，低 SOC 和高 SOC 土壤的真菌群落组成存在显著差异（p<0.05）。在低 SOC 土壤中，随着培养时间的延长，子囊菌门的相对丰度逐渐增加，而腔菌门和散囊菌门的相对丰度逐渐减少。相反，高 SOC 土壤中的真菌群落相对稳定。在低和高 SOC 含量土壤中，子囊菌门和腔菌门的相对丰度与 PCB 降解呈正相关。研究结果表明，SOC 含量影响生物炭添加土壤中 PCB 的降解和真菌群落组成，生物炭对低 SOC 含量土壤中 PCB 具有较高的修复潜力。
