Hu S I, Miller S M, Samols D
Biochemistry. 1986 Dec 2;25(24):7834-9. doi: 10.1021/bi00372a008.
C-reactive protein (CRP), an acute-phase plasma protein of hepatic origin in man and rabbit, is a cyclic pentamer composed of five identical nonglycosylated Mr 22 500 subunits. We have isolated both cDNA and genomic clones for rabbit CRP. These clones were used as probes to demonstrate that when CRP synthesis is increased following an acute inflammatory stimulus, there is a corresponding increase in the level of accumulated CRP mRNA. The rabbit CRP gene is 2.6 kilobases in length containing a single intron of 252 base pairs (bp) which interrupts the codon for amino acid 2 in the protein. The mRNA for CRP contains a 5'-nontranslated region of 113 bp and a 3'-nontranslated region of 1550 bp. Sequencing of the protein-coding region of the gene indicates that the primary translation product contains a 20 amino acid N-terminal signal peptide. The deduced amino acid sequence is in general agreement with the published sequence [Wang, C. M., Nguyen, N. Y., Yonaha, K., Robey, F., & Liu, T.-Y. (1982) J. Biol. Chem. 257, 13610-13615] except in the region between amino acids 63 and 73. In this region, the sequence of both cDNA and genomic clones indicates the presence of 28 amino acids not previously reported. This alteration may be the result of genetic heterogeneity or an error in the reported protein sequence.
C反应蛋白（CRP）是人和兔肝脏来源的一种急性期血浆蛋白，是由五个相同的非糖基化、分子量为22500的亚基组成的环状五聚体。我们已经分离出兔CRP的cDNA和基因组克隆。这些克隆用作探针，以证明在急性炎症刺激后CRP合成增加时，积累的CRP mRNA水平相应增加。兔CRP基因长度为2.6千碱基，含有一个252个碱基对（bp）的单一内含子，该内含子打断了蛋白质中第2个氨基酸的密码子。CRP的mRNA包含一个113bp的5'非翻译区和一个1550bp的3'非翻译区。对该基因蛋白质编码区的测序表明，初级翻译产物包含一个20个氨基酸的N端信号肽。推导的氨基酸序列与已发表的序列[Wang, C. M., Nguyen, N. Y., Yonaha, K., Robey, F., & Liu, T.-Y. (1982) J. Biol. Chem. 257, 13610-13615]总体一致，除了在氨基酸63和73之间的区域。在该区域，cDNA和基因组克隆的序列均表明存在28个以前未报道的氨基酸。这种改变可能是遗传异质性的结果，或者是报道的蛋白质序列中的一个错误。
