[β-内啡肽和骨髓肽对体外淋巴细胞环磷酸腺苷水平及增殖的影响]

[Effect of beta-endorphin and myelopeptides on the cAMP level and proliferation of lymphocytes in vitro].

作者信息

Zozulia A A, Patsakova E, Kost N V, Ivanushkin A M, Voronkova T L

出版信息

Biull Eksp Biol Med. 1986 Dec;102(12):731-3.

Abstract

Beta-endorphin (10(-11)-10(-9) M) has been shown to induce naloxone-independent depression of the proliferative activity of human peripheral lymphocytes (HL), stimulated by pokeweed mitogen without affecting PHA-stimulated HL proliferation. Beta-endorphin (10(-10)-10(-7) M) also caused changes in HL cAMP level, that were blocked by naloxone. Marked individual sensitivity to beta-endorphin effects has been noted. It has been also shown that a bone marrow preparation, stimulating antibody production (myelopeptides), causes naloxone-independent depression in the proliferative activity of HL, stimulated by PHA and pokeweed mitogen, as well as naloxone-blocked decrease in cAMP HL level. It has been concluded that beta-endorphin interacts with several types of opiate lymphocyte receptors and that opioids, contained in myelopeptides, are involved in the realization of myelopeptide effect on lymphocytes.

摘要

β-内啡肽（10⁻¹¹ - 10⁻⁹ M）已被证明可诱导人外周淋巴细胞（HL）增殖活性出现纳洛酮非依赖性抑制，此抑制作用由商陆有丝分裂原刺激产生，且不影响PHA刺激的HL增殖。β-内啡肽（10⁻¹⁰ - 10⁻⁷ M）还会引起HL细胞中cAMP水平的变化，这种变化可被纳洛酮阻断。已观察到个体对β-内啡肽效应存在显著敏感性。研究还表明，一种刺激抗体产生的骨髓制剂（骨髓肽）可导致PHA和商陆有丝分裂原刺激的HL增殖活性出现纳洛酮非依赖性抑制，以及HL细胞中cAMP水平出现纳洛酮阻断的降低。由此得出结论，β-内啡肽与多种类型的阿片类淋巴细胞受体相互作用，且骨髓肽中所含的阿片类物质参与了骨髓肽对淋巴细胞作用的实现。