Newport J, Spann T
Cell. 1987 Jan 30;48(2):219-30. doi: 10.1016/0092-8674(87)90425-9.
We describe a stable cell-free mitotic extract derived from Xenopus eggs that contains activities necessary for nuclear envelope breakdown and chromosome condensation during mitosis. Using these cell-free extracts, we have demonstrated that nuclear envelope vesicularization, lamina solubilization, and chromosome condensation are independent and separable biochemical processes. We present evidence indicating that during mitosis nuclear membrane breakdown may involve the binding of a coating protein, lamin solubilization is enzymatically driven, and chromosome condensation involves both binding proteins and enzymatic activities including topoisomerase II. These results provide a coherent framework for investigating structural modification of the nucleus during mitosis at the biochemical level.
我们描述了一种从非洲爪蟾卵中获得的稳定无细胞有丝分裂提取物，其含有有丝分裂期间核膜破裂和染色体凝聚所需的活性。利用这些无细胞提取物，我们证明了核膜囊泡化、核纤层溶解和染色体凝聚是独立且可分离的生化过程。我们提供的证据表明，在有丝分裂期间，核膜破裂可能涉及一种包被蛋白的结合，核纤层溶解是由酶驱动的，而染色体凝聚则涉及结合蛋白和包括拓扑异构酶II在内的酶活性。这些结果为在生化水平上研究有丝分裂期间细胞核的结构修饰提供了一个连贯的框架。
