Dosquet-Bernard C, Wilhelm F, Lomri N, Tobelem G, Caen J P
Cell Biol Int Rep. 1986 Dec;10(12):931-8. doi: 10.1016/0309-1651(86)90113-x.
We investigated the effect of 1 alpha,25-dihydroxyvitamin D3 (1,25 (OH)2 vit D3) on the 3H-thymidine uptake by Balb/c 3T3 cells and by human skin fibroblasts stimulated by normal human serum or by purified PDGF. We found an inhibitory effect of 1,25 (OH)2 vit D3 on the DNA synthesis of Balb/c 3T3 cells grown in the presence of human serum as well as in the presence of PDGF. At 5% human serum this effect is minimal at 10(-12) M 1,25 (OH)2 vit D3 and is maximal at 10(-9) M. On the DNA synthesis of human fibroblasts stimulated by human serum or by PDGF a modulatory effect of 1,25 (OH)2 vit D3 was shown. On these cells the vitamin had a stimulatory effect between 10(-11) and 10(-9) M and an inhibitory effect at very high concentrations (10(-7) M). Our results suggested that the effect of 1,25 (OH)2 vit D3 on fibroblast DNA synthesis could be mediated by interactions with its specific intracellular receptor. 1,25 (OH)2 vit D3 had no any action on the growth of human fibroblasts stimulated by fibroblast growth factor.
我们研究了1α,25 - 二羟基维生素D3（1,25(OH)2维生素D3）对Balb/c 3T3细胞以及受正常人血清或纯化血小板衍生生长因子（PDGF）刺激的人皮肤成纤维细胞摄取3H - 胸腺嘧啶核苷的影响。我们发现1,25(OH)2维生素D3对在人血清存在以及PDGF存在下生长的Balb/c 3T3细胞的DNA合成具有抑制作用。在5%人血清条件下，这种作用在10^(-12)M 1,25(OH)2维生素D3时最小，在10^(-9)M时最大。对于受血清或PDGF刺激的人成纤维细胞的DNA合成，1,25(OH)2维生素D3显示出调节作用。在这些细胞上，该维生素在10^(-11)至10^(-9)M之间具有刺激作用，在非常高的浓度（10^(-7)M）时具有抑制作用。我们的结果表明，1,25(OH)2维生素D3对成纤维细胞DNA合成的作用可能是通过与其特异性细胞内受体相互作用介导的。1,25(OH)2维生素D3对受成纤维细胞生长因子刺激的人成纤维细胞的生长没有任何作用。
