Chung F Z, Lentes K U, Gocayne J, Fitzgerald M, Robinson D, Kerlavage A R, Fraser C M, Venter J C
FEBS Lett. 1987 Jan 26;211(2):200-6. doi: 10.1016/0014-5793(87)81436-9.
Two cDNA clones, lambda-CLFV-108 and lambda-CLFV-119, encoding for the beta-adrenergic receptor, have been isolated from a human brain stem cDNA library. One human genomic clone, LCV-517 (20 kb), was characterized by restriction mapping and partial sequencing. The human brain beta-receptor consists of 413 amino acids with a calculated Mr of 46480. The gene contains three potential glucocorticoid receptor-binding sites. The beta-receptor expressed in human brain was homology with rodent (88%) and avian (52%) beta-receptors and with porcine muscarinic cholinergic receptors (31%), supporting our proposal [(1984) Proc. Natl. Acad. Sci. USA 81, 272 276] that adrenergic and muscarinic cholinergic receptors are structurally related. This represents the first cloning of a neurotransmitter receptor gene from human brain.
已从人脑干cDNA文库中分离出两个编码β-肾上腺素能受体的cDNA克隆，λ-CLFV-108和λ-CLFV-119。通过限制性酶切图谱分析和部分测序对一个人基因组克隆LCV-517（20kb）进行了表征。人脑中的β-受体由413个氨基酸组成，计算分子量为46480。该基因包含三个潜在的糖皮质激素受体结合位点。在人脑中表达的β-受体与啮齿动物（88%）和禽类（52%）的β-受体以及猪毒蕈碱胆碱能受体（31%）具有同源性，这支持了我们之前的提议[（1984年）《美国国家科学院院刊》81, 272 - 276]，即肾上腺素能和毒蕈碱胆碱能受体在结构上相关。这是首次从人脑中克隆神经递质受体基因。
