Nies B A, Meyer J F, Wiedemann B
J Antimicrob Chemother. 1986 Oct;18 Suppl C:35-41. doi: 10.1093/jac/18.supplement_c.35.
The multi-resistance plasmid pBP16 was used to analyse the variability of R-factors from clinical isolates and the molecular structures and processes involved. The observed rearrangement in pBP16 and its derivatives included inversion, deletion, replicon fusion and dissociation, and transposition. All these events could be traced to the presence and activity of multiple copies of the IS-element IS160 within pBP16. Since IS-elements are common in R-factors, it is likely that they are one of the main reasons for plasmid instability and that they are involved in a major way in plasmid evolution.
多抗性质粒pBP16用于分析临床分离株中R因子的变异性以及所涉及的分子结构和过程。在pBP16及其衍生物中观察到的重排包括倒位、缺失、复制子融合和解离以及转座。所有这些事件都可追溯到pBP16内IS元件IS160多个拷贝的存在和活性。由于IS元件在R因子中很常见，它们很可能是质粒不稳定的主要原因之一，并且在质粒进化中起主要作用。
