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大肠杆菌K-12的rho突变体中转座减少。

Reduced transposition in rho mutants of Escherichia coli K-12.

作者信息

Datta A R, Rosner J L

出版信息

J Bacteriol. 1987 Feb;169(2):888-90. doi: 10.1128/jb.169.2.888-890.1987.

DOI:10.1128/jb.169.2.888-890.1987
PMID:3027052
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211863/
Abstract

Substantially reduced frequencies of transposition for the transposons Tn5 and Tn9 and the insertion sequences IS1 and IS5 were observed in several rho mutants of Escherichia coli K-12 compared with those observed in their isogenic wild-type counterparts. The lower transposition frequencies could be due to decreased supercoiling of DNA, to altered expression of required genes, or to aberrant transcription of transposon or target DNA resulting from the lack of transcription termination at Rho-sensitive sites in rho mutants.

摘要

与同基因野生型大肠杆菌K-12相比，在几种rho突变体中观察到转座子Tn5和Tn9以及插入序列IS1和IS5的转座频率大幅降低。转座频率降低可能是由于DNA超螺旋减少、所需基因表达改变，或者是由于rho突变体中Rho敏感位点缺乏转录终止导致转座子或靶DNA转录异常。

相似文献

1
Reduced transposition in rho mutants of Escherichia coli K-12.大肠杆菌K-12的rho突变体中转座减少。
J Bacteriol. 1987 Feb;169(2):888-90. doi: 10.1128/jb.169.2.888-890.1987.
2
[Mutants of Escherichia coli K-12 with altered excision efficiency of transposons Tn5 and Tn9].[转座子Tn5和Tn9切除效率改变的大肠杆菌K-12突变体]
Genetika. 1989 Apr;25(4):605-13.
3
[Evidence for Campbell's mechanism for fine excision of Tn9 type transposons in Escherichia coli K12].[大肠杆菌K12中Tn9型转座子精细切除的坎贝尔机制的证据]
Mol Biol (Mosk). 1991 May-Jun;25(3):614-23.
4
Artificial transposable elements in the study of the ends of IS1.用于研究IS1末端的人工转座元件。
Gene. 1987;61(1):91-101. doi: 10.1016/0378-1119(87)90368-4.
5
[Characteristics of Escherichia coli K-12 mutants with altered effectiveness of excision of Tn5 and Tn9 transposons].[具有改变的Tn5和Tn9转座子切除效率的大肠杆菌K-12突变体的特征]
Genetika. 1989 May;25(5):829-37.
6
[Tn9 integration sites and their effect on transposon properties].[Tn9整合位点及其对转座子特性的影响]
Genetika. 1980;16(1):46-54.
7
[Study of the role of the insA open reading frame in the IS1 insertion element structure during transposition and resolution of the IS1 mediated cointegrates].[关于insA开放阅读框在IS1介导的共整合体转座和拆分过程中在IS1插入元件结构中的作用的研究]
Mol Biol (Mosk). 1990 Nov-Dec;24(6):1549-61.
8
Genetic evidence for absence of transposition functions from the internal part of Tn981 a relative of Tn9.Tn981（Tn9的一个亲缘基因）内部缺失转座功能的遗传学证据。
Mol Gen Genet. 1980;177(4):667-74. doi: 10.1007/BF00272678.
9
[Genetic study of Escherichia coli K-12 mutations that affect the transposition process].[影响转座过程的大肠杆菌K-12突变的遗传学研究]
Genetika. 1981;17(1):33-44.
10
The pleiotropic ts15 mutation of E. coli is an IS1 insertion in the rho structural gene.大肠杆菌的多效性ts15突变是IS1插入rho结构基因中。
Genetics. 1983 Oct;105(2):265-80. doi: 10.1093/genetics/105.2.265.

引用本文的文献

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Investigating How Genomic Contexts Impact IS5 Transposition Within the Genome.研究基因组背景如何影响基因组内IS5转座
Microorganisms. 2024 Dec 16;12(12):2600. doi: 10.3390/microorganisms12122600.
2
Mastering the control of the Rho transcription factor for biotechnological applications.掌握 Rho 转录因子的控制用于生物技术应用。
Appl Microbiol Biotechnol. 2021 May;105(10):4053-4071. doi: 10.1007/s00253-021-11326-7. Epub 2021 May 8.
3
Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase.鉴定大肠杆菌K-12的ybhE基因即为pgl，其编码6-磷酸葡萄糖酸内酯酶。
J Bacteriol. 2004 Dec;186(24):8248-53. doi: 10.1128/JB.186.24.8248-8253.2004.
4
Target site choice of the related transposable elements Tc1 and Tc3 of Caenorhabditis elegans.秀丽隐杆线虫相关转座元件Tc1和Tc3的靶位点选择
Nucleic Acids Res. 1994 Feb 11;22(3):262-9. doi: 10.1093/nar/22.3.262.
5
A late exclusion of bacteriophage T4 can be suppressed by Escherichia coli GroEL or Rho.大肠杆菌的GroEL或Rho可以抑制噬菌体T4的晚期排除。
Genetics. 1994 Jul;137(3):613-25. doi: 10.1093/genetics/137.3.613.
6
Transcription of the target is required for IS102 mediated deletions.IS102介导的缺失需要靶标的转录。
Mol Gen Genet. 1988 May;212(2):265-70. doi: 10.1007/BF00334695.
7
Expression of proteins essential for IS1 transposition: specific binding of InsA to the ends of IS1.IS1转座所需蛋白质的表达：InsA与IS1末端的特异性结合。
EMBO J. 1987 Oct;6(10):3163-9. doi: 10.1002/j.1460-2075.1987.tb02627.x.

本文引用的文献

1
A deletion analysis of transposon Tn9.转座子Tn9的缺失分析。
J Mol Biol. 1984 Mar 15;173(4):523-9. doi: 10.1016/0022-2836(84)90395-4.
2
Escherichia coli K-12 gyrB gene product is involved in the lethal effect of the ligts2 mutant of bacteriophage Mu.大肠杆菌K-12 gyrB基因产物参与噬菌体Mu的ligts2突变体的致死效应。
J Bacteriol. 1984 Feb;157(2):665-8. doi: 10.1128/jb.157.2.665-668.1984.
3
In vitro transposition of bacteriophage Mu: a biochemical approach to a novel replication reaction.噬菌体Mu的体外转座：一种针对新型复制反应的生化方法。
Cell. 1983 Dec;35(3 Pt 2):785-94. doi: 10.1016/0092-8674(83)90111-3.
4
Cointegrate formation mediated by Tn9. II. Activity of IS1 is modulated by external DNA sequences.由Tn9介导的共整合体形成。II. IS1的活性受外部DNA序列调控。
J Mol Biol. 1983 Oct 15;170(1):61-91. doi: 10.1016/s0022-2836(83)80227-7.
5
The pleiotropic ts15 mutation of E. coli is an IS1 insertion in the rho structural gene.大肠杆菌的多效性ts15突变是IS1插入rho结构基因中。
Genetics. 1983 Oct;105(2):265-80. doi: 10.1093/genetics/105.2.265.
6
Repression of cointegration ability of insertion element IS1 by transcriptional readthrough from flanking regions.侧翼区域转录通读对插入元件IS1共整合能力的抑制作用。
Cell. 1983 Aug;34(1):135-42. doi: 10.1016/0092-8674(83)90143-5.
7
Detection of chemicals that stimulate Tn9 transposition in Escherichia coli K12.在大肠杆菌K12中刺激Tn9转座的化学物质的检测。
Mol Gen Genet. 1983;189(2):245-50. doi: 10.1007/BF00337812.
8
Control of transposon Tn5 transposition in Escherichia coli.大肠杆菌中转座子Tn5转座的控制
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7450-4. doi: 10.1073/pnas.79.23.7450.
9
DNA gyrase is a host factor required for transposition of Tn5.DNA 回旋酶是 Tn5 转座所需的一种宿主因子。
Cell. 1982 Aug;30(1):9-18. doi: 10.1016/0092-8674(82)90006-x.
10
A new class of mutants in DNA polymerase I that affects gene transposition.DNA聚合酶I中一类影响基因转座的新型突变体。
J Mol Biol. 1982 Jun 25;158(2):203-12. doi: 10.1016/0022-2836(82)90429-6.