Key Laboratory of Photochemistry Biomaterials and Energy Storage Materials of Heilongjiang Province, College of Chemistry & Chemical Engineering, Harbin Normal University, No.1 South Road, Limin Development Zone, Harbin 150025, China.
College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang 154007, China.
Molecules. 2018 Sep 30;23(10):2514. doi: 10.3390/molecules23102514.
Xiaoyao Powder (XYP), a common Chinese medicine, comprises eight traditional Chinese herbs and has been widely used clinically to treat liver damage and mental disorders. An ultra-performance liquid chromatography⁻tandem mass spectrometry method was developed to investigate the pharmacokinetics of 14 compounds (albiflorin, paeoniflorin, ferulic acid, senkyunolide I, quercetin, isoliquiritigenin, atractylenolide III, ligustilide, atractylenolide II, liquiritin, liquiritigenin, saikosaponin c, glycyrrhizic acid, and saikosaponin a) in XYP. Naringenin was used as the internal standard. The compounds were separated using an ACQUITY UPLC BEH C18 column (1.7 μm, 50 × 2.1 mm) with a mobile phase consisting of acetonitrile and 0.1% formic acid in water at a flow rate of 0.3 mL/min. Detection was performed on a triple-quadrupole tandem mass spectrometer using multiple reaction monitoring and an electrospray ionization source in both positive and negative ionization modes. All calibration curves exhibited good linearity (r² > 0.9974) over the measured ranges. The intra- and inter-day precisions were within 12%, and the accuracy ranged from 89.93% to 106.64%. Extraction recovery and matrix effect results were satisfactory. The method was successfully applied in a pharmacokinetic study of the 14 compounds in rat plasma after the oral administration of XYP.
逍遥散（XYP）是一种常见的中药，由八种中药组成，已广泛应用于临床治疗肝损伤和精神障碍。建立了一种超高效液相色谱⁻串联质谱法用于研究逍遥散中 14 种化合物（白芍苷、丹皮酚、阿魏酸、升麻素 I、槲皮素、异甘草素、阿魏酸内酯 III、藁本内酯、阿魏酸内酯 II、甘草苷、甘草素、柴胡皂苷 c、甘草酸和柴胡皂苷 a）的药代动力学。柚皮苷作为内标。采用 ACQUITY UPLC BEH C18 柱（1.7μm，50×2.1mm），以乙腈和 0.1%甲酸水溶液为流动相，流速为 0.3mL/min，对化合物进行分离。采用电喷雾源正、负离子模式下的多重反应监测，对三重四极杆串联质谱进行检测。所有校准曲线在测定范围内均表现出良好的线性（r²＞0.9974）。日内和日间精密度均在 12%以内，准确度在 89.93%至 106.64%之间。提取回收率和基质效应结果令人满意。该方法成功应用于大鼠口服逍遥散后血浆中 14 种化合物的药代动力学研究。
