Department of Chemistry, Science Institute, Dunhaga 3, 107 Reykjavik, Iceland.
Chem Commun (Camb). 2018 Oct 16;54(83):11749-11752. doi: 10.1039/c8cc05597a.
In the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) electron paramagnetic resonance (EPR), pulsed electron-electron double resonance (PELDOR) and fluorescence spectroscopies.
在未经修饰的 RNA 定点自旋标记的第一个实例中，通过连续波（CW）电子顺磁共振（EPR）、脉冲电子-电子双共振（PELDOR）和荧光光谱学测定，表明四甲基罗丹明（TMR）的吡咯烷-氮氧化物衍生物与孔雀石绿（MG）适体具有高亲和力。
