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A bacterial protein requirement for the bacteriophage lambda terminase reaction.

作者信息

Gold M, Parris W

出版信息

Nucleic Acids Res. 1986 Dec 22;14(24):9797-809. doi: 10.1093/nar/14.24.9797.

DOI:10.1093/nar/14.24.9797
PMID:3027664
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC341336/
Abstract

The bacteriophage lambda terminase enzyme cleaves the cohesive-end sites of lambda DNA to yield the protruding 5'-termini of the mature molecule. In vitro, this endonucleolytic event requires a protein factor which has been isolated and purified from extracts of uninfected E. coli. The terminase host factor (THF) is a heat stable basic protein of M.W. approximately 22,000. The integration host factor (IHF) protein of E. coli can efficiently substitute for THF in the terminase reaction; however, THF can be demonstrated to be physically present in, and isolated with full biological activity from extracts of cells defective or deficient in IHF.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/99fb8aacf92a/nar00293-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/1f96fe9723e0/nar00293-0257-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/5f3483120815/nar00293-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/affce5709d47/nar00293-0259-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/220c52f987c4/nar00293-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/06d0ad897493/nar00293-0263-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/99fb8aacf92a/nar00293-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/1f96fe9723e0/nar00293-0257-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/5f3483120815/nar00293-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/affce5709d47/nar00293-0259-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/220c52f987c4/nar00293-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/06d0ad897493/nar00293-0263-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31cc/341336/99fb8aacf92a/nar00293-0264-a.jpg

相似文献

1
A bacterial protein requirement for the bacteriophage lambda terminase reaction.
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2
Essential interaction between lambdoid phage 21 terminase and the Escherichia coli integrative host factor.
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3
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J Bacteriol. 1991 Mar;173(5):1670-6. doi: 10.1128/jb.173.5.1670-1676.1991.
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J Mol Biol. 1993 Mar 20;230(2):492-504. doi: 10.1006/jmbi.1993.1166.
10
Analysis of cosmids using linearization by phage lambda terminase.利用噬菌体λ末端酶线性化分析黏粒。
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引用本文的文献

1
Lethal effect of lambda DNA terminase in recombination deficient Escherichia coli.λ噬菌体DNA末端酶在重组缺陷型大肠杆菌中的致死效应
Mol Gen Genet. 1988 Jul;213(1):42-9. doi: 10.1007/BF00333396.
2
Bacteriophage lambda DNA packaging: a mutant terminase that is independent of integration host factor.噬菌体λ DNA 包装：一种不依赖整合宿主因子的突变型末端酶。
Mol Gen Genet. 1988 Apr;212(1):142-8. doi: 10.1007/BF00322457.
3
The interaction of Escherichia coli integration host factor with the cohesive end sites of phages lambda and 21.

本文引用的文献

1
Novel histone H2A-like protein of escherichia coli.大肠杆菌的新型组蛋白H2A样蛋白。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5097-101. doi: 10.1073/pnas.77.9.5097.
2
Integration and excision of bacteriophage lambda: the mechanism of conservation site specific recombination.噬菌体λ的整合与切除：保守位点特异性重组机制
Annu Rev Genet. 1981;15:143-67. doi: 10.1146/annurev.ge.15.120181.001043.
3
int-h: An int mutation of phage lambda that enhances site-specific recombination.int-h：噬菌体λ的一种int突变，可增强位点特异性重组。
大肠杆菌整合宿主因子与噬菌体λ和21的粘性末端位点的相互作用。
Nucleic Acids Res. 1988 Mar 25;16(5):2015-30. doi: 10.1093/nar/16.5.2015.
4
The bacteriophage lambda cohesive end site: isolation of spacing/substitution mutations that result in dependence on Escherichia coli integration host factor.λ噬菌体粘性末端位点：导致对大肠杆菌整合宿主因子依赖的间隔/取代突变的分离
Mol Gen Genet. 1988 Apr;212(1):157-65. doi: 10.1007/BF00322459.
5
A point mutation in the Nul gene of bacteriophage lambda facilitates phage growth in Escherichia coli with himA and gyrB mutations.噬菌体λ的Nul基因中的一个点突变促进了噬菌体在具有himA和gyrB突变的大肠杆菌中的生长。
Mol Gen Genet. 1988 Apr;212(1):149-56. doi: 10.1007/BF00322458.
6
Terminase host factor: a histone-like E. coli protein which can bind to the cos region of bacteriophage lambda DNA.末端酶宿主因子：一种类组蛋白的大肠杆菌蛋白，可与噬菌体λ DNA的粘性末端区域结合。
Nucleic Acids Res. 1988 Apr 11;16(7):2765-85. doi: 10.1093/nar/16.7.2765.
7
The Nul subunit of bacteriophage lambda terminase binds to specific sites in cos DNA.噬菌体λ末端酶的Nul亚基与粘性末端（cos）DNA中的特定位点结合。
J Virol. 1988 Feb;62(2):387-92. doi: 10.1128/JVI.62.2.387-392.1988.
8
Integration host factor (IHF) stimulates binding of the gpNu1 subunit of lambda terminase to cos DNA.整合宿主因子（IHF）刺激λ末端酶的gpNu1亚基与粘性末端位点（cos）DNA的结合。
Nucleic Acids Res. 1989 Mar 11;17(5):2005-22. doi: 10.1093/nar/17.5.2005.
9
The interaction of E. coli integration host factor and lambda cos DNA: multiple complex formation and protein-induced bending.大肠杆菌整合宿主因子与λ cos DNA的相互作用：多种复合物的形成及蛋白质诱导的弯曲
Nucleic Acids Res. 1989 Jan 11;17(1):317-34. doi: 10.1093/nar/17.1.317.
10
Bacteriophage lambda DNA: the beginning of the end.λ噬菌体DNA：终结的开端。
J Bacteriol. 1990 Jun;172(6):2819-24. doi: 10.1128/jb.172.6.2819-2824.1990.
Cell. 1980 Jul;20(3):721-9. doi: 10.1016/0092-8674(80)90318-9.
4
Isolation and characterization of nucleoid proteins from Escherichia coli.大肠杆菌类核蛋白的分离与特性分析
Mol Gen Genet. 1984;196(2):217-24. doi: 10.1007/BF00328053.
5
The bacteriophage lambda terminase. Partial purification and preliminary characterization of properties.λ噬菌体末端酶。部分纯化及性质的初步表征。
J Biol Chem. 1983 Dec 10;258(23):14619-25.
6
Purification and characterization of a protein from Escherichia coli which forms complexes with superhelical and single-stranded DNAs.从大肠杆菌中纯化和鉴定一种能与超螺旋DNA和单链DNA形成复合物的蛋白质。
J Biochem. 1982 Oct;92(4):1059-68. doi: 10.1093/oxfordjournals.jbchem.a134021.
7
Circular monomers of bacteriophage lambda DNA as substrates for in vitro packaging.
Virology. 1982 May;119(1):35-41. doi: 10.1016/0042-6822(82)90062-9.
8
Purification and properties of the Escherichia coli protein factor required for lambda integrative recombination.λ整合重组所需的大肠杆菌蛋白质因子的纯化及特性
J Biol Chem. 1981 Sep 10;256(17):9246-53.
9
DNA packaging in the lambdoid phages: the role of lambda genes Nu1 and A.
Virology. 1981 Jun;111(2):642-6. doi: 10.1016/0042-6822(81)90363-9.
10
Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.从十二烷基硫酸钠 - 聚丙烯酰胺凝胶中洗脱蛋白质、去除十二烷基硫酸钠以及酶活性的复性：大肠杆菌RNA聚合酶σ亚基、小麦胚芽DNA拓扑异构酶及其他酶的实验结果
Anal Biochem. 1980 Nov 15;109(1):76-86. doi: 10.1016/0003-2697(80)90013-5.