蛇毒蛋白质组学在体内抗蛇毒血清评估中的功能应用
Functional Application of Snake Venom Proteomics in In Vivo Antivenom Assessment.
作者信息
Tan Choo Hock, Tan Kae Yi
机构信息
Venom Research and Toxicology Laboratory, Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Protein and Interactomic Laboratory, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
出版信息
Methods Mol Biol. 2019;1871:153-158. doi: 10.1007/978-1-4939-8814-3_11.
Reverse-phase high-performance liquid chromatography is commonly employed as a decomplexing strategy in snake venom proteomics. The chromatographic fractions often contain relatively pure toxins that can be assessed functionally for toxicity level through the determination of their median lethal doses (LD). Further, antivenom efficacy can be evaluated specifically against these venom fractions to understand the limitation of the antivenom as the treatment for snake envenomation. However, methods of toxicity assessment and antivenom evaluation vary across laboratories; hence there is a need to standardize the protocols and parameters, in particular those related to the neutralizing efficacy of antivenom. This chapter outlines the important in vivo techniques and data interpretation that can be applied in the functional study of snake venom proteomes.
反相高效液相色谱法通常被用作蛇毒蛋白质组学中的一种解络合策略。色谱级分通常含有相对纯净的毒素，可通过测定其半数致死剂量（LD）在功能上评估其毒性水平。此外，可以针对这些毒液级分专门评估抗蛇毒血清的疗效，以了解抗蛇毒血清作为蛇咬伤治疗方法的局限性。然而，不同实验室的毒性评估和抗蛇毒血清评估方法各不相同；因此，有必要对方案和参数进行标准化，特别是那些与抗蛇毒血清中和效力相关的参数。本章概述了可应用于蛇毒蛋白质组功能研究的重要体内技术和数据解读方法。
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