Albrecht-Buehler G
Scan Electron Microsc. 1986(Pt 4):1427-36.
Using metallic gold in various assays for the motility of cultured tissue cells, the paper compares the movements of surface projections and the locomotion of polyoma (Py3T3) and SV40 (SV3T3) virus-transformed 3T3 cells with the behavior of the parental 3T3 cells. The movement of surface projections was assayed by the ability of filopodia, lamellipodia and blebs of freshly plated cells to remove colloidal gold particles from a particle-coated glass substrate. The ability of filopodia to probe the environment for points of anchorage was tested by observing cells plated on glass whose filopodia touched the surface of a neighboring area of evaporated gold. The locomotion of cells was assayed by particle-free tracks (phagokinetic tracks) which were left by migrating cells on a glass substrate which was previously coated with colloidal gold particles. The paper suggests that the ability of the transformed cells to sense environmental factors, and their behavioral controls are altered.
在各种用于培养组织细胞运动性的检测中使用金属金，本文比较了表面突起的运动以及多瘤病毒（Py3T3）和SV40病毒（SV3T3）转化的3T3细胞的移动与亲代3T3细胞的行为。通过新鲜铺板细胞的丝状伪足、片状伪足和泡状突起从颗粒包被的玻璃基质上去除胶体金颗粒的能力来检测表面突起的运动。通过观察铺在玻璃上的细胞，其丝状伪足接触到相邻蒸发金区域的表面，来测试丝状伪足探测环境中附着点的能力。细胞的移动通过无颗粒轨迹（吞噬运动轨迹）来检测，这些轨迹是迁移细胞在先前涂有胶体金颗粒的玻璃基质上留下的。本文表明，转化细胞感知环境因素的能力及其行为控制发生了改变。
