Department of Neurology and Neurobiology of Aging, Kanazawa University Graduate School of Medical Science, 13-1 Takara-machi, Kanazawa 920-8640, Japan.
Health Service Center, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
J Neuroimmunol. 2018 Dec 15;325:74-78. doi: 10.1016/j.jneuroim.2018.09.012. Epub 2018 Sep 27.
We investigated the expression patterns of programmed cell death-ligand 1 (PD-L1) in the skeletal muscle of 15 patients with myasthenia gravis (MG). In 11 of the patients, immunohistochemistry confirmed the localization of PD-L1 in the muscle cell membrane with cytoplasmic integrity. PD-L1 mRNA expression levels were higher in the muscles of MG patients as compared to those of non-myogenic patients (p < .05). Moreover, a unimodal relationship between PD-L1 mRNA levels and quantitative MG scores for disease severity was identified by a generalized additive model, indicating reciprocal effects. Together, these results suggest that MG symptoms might influence PD-L1 expression in muscle cells, and upregulated PD-L1 expression might inhibit autoimmune reactivity.
我们研究了程序性细胞死亡配体 1（PD-L1）在 15 名重症肌无力（MG）患者骨骼肌中的表达模式。在 11 名患者中，免疫组织化学证实 PD-L1 定位于具有细胞质完整性的肌细胞膜上。与非肌源性患者相比，MG 患者肌肉中的 PD-L1 mRNA 表达水平更高（p <.05）。此外，广义加性模型确定了 PD-L1 mRNA 水平与定量 MG 评分之间的单峰关系，表明存在相互作用。总之，这些结果表明 MG 症状可能会影响肌肉细胞中的 PD-L1 表达，而上调的 PD-L1 表达可能会抑制自身免疫反应。
