De Keyser Kirsten, Dierick Noël, Kanto Uthai, Hongsapak Tassanan, Buyens Goedele, Kuterna Leni, Vanderbeke Erik
AVEVE Biochem NV, Merksem, Belgium.
Department of Animal Sciences and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
J Anim Physiol Anim Nutr (Berl). 2019 Jan;103(1):221-230. doi: 10.1111/jpn.12998. Epub 2018 Oct 2.
The influence of medium-chain glycerides on performance and gastrointestinal well-being in weaning piglets was assessed. First, caproic (C6), caprylic (C8) and capric (C10) acid activity against Escherichia coli was screened in vitro. Pig flora of the whole small intestine was used as inoculum. Seven in vitro incubations were done in duplicate at pH = 3 and 5: C10 (15 mM), C8 (12 mM), C6 (15, 12, 10 mM), a non-incubated-negative control and incubated negative control. Culture suspensions were plated on E. coli-selective agar. Controls showed bacterial growth. C6 and C8 showed no growth at both pH-values, where C10 showed growth at pH = 5. Secondly, an in vivo study was done with 80 weaned piglets over 42 days, housed in pens of eight animals (five pens/treatment), fed a basal diet containing broken rice/soya bean meal/fish meal and supplemented with C6 and C8 in medium-chain glyceride form (MCT6/8, 0.175%) or antibiotic growth promoter (AGP, 0.020%) (Kasetsart University, Thailand) serving as control. Feed intake, daily gain and feed-to-gain ratio did not differ between MCT6/8 and AGP. Per replicate, two random selected piglets were challenged intravenously with E. coli-lipopolysaccharide (LPS) or saline solution (S) at Days 21 and 28. All challenged animals were sacrificed; blood and digestive tract samples (jejunum/ileum) were collected at Day 35. LPS challenge consistently reduced villus height and crypt depth for MCT6/8 and AGP. However, LPS-challenged piglets supplemented with MCT6/8 restored villus height, where AGP did not. MCT6/8 piglets had higher serum IgA, more jejunal IgA-positive plasma cells and goblet cells than AGP. At the ileal level, results were similar, though less pronounced. The present study offers new insight in the benefits of MCT6/8 over AGP in the post-weaning period. There is in vitro anti-microbial action of C6 and C8 on E. coli. In vivo, MCT6/8 also has protective effects in the small intestine that may result in growth promotion.
评估了中链甘油酯对断奶仔猪生产性能和胃肠道健康的影响。首先，在体外筛选了己酸（C6）、辛酸（C8）和癸酸（C10）对大肠杆菌的活性。使用整个小肠的猪肠道菌群作为接种物。在pH = 3和5的条件下进行了七次体外培养，一式两份：C10（15 mM）、C8（12 mM）、C6（15、12、10 mM）、未培养的阴性对照和培养的阴性对照。将培养悬浮液接种于大肠杆菌选择性琼脂平板上。对照显示有细菌生长。C6和C8在两个pH值下均未生长，而C10在pH = 5时生长。其次，对80头断奶仔猪进行了为期42天的体内研究，每栏饲养8头仔猪（每个处理5栏），饲喂含碎米/豆粕/鱼粉的基础日粮，并添加中链甘油酯形式的C6和C8（MCT6/8，0.175%）或抗生素生长促进剂（AGP，0.020%）（泰国 Kasetsart 大学）作为对照。MCT6/8组和AGP组的采食量、日增重和料重比没有差异。在第21天和第28天，每个重复栏中随机选择两头仔猪静脉注射大肠杆菌脂多糖（LPS）或生理盐水（S）。对所有受挑战的动物实施安乐死；在第35天采集血液和消化道样本（空肠/回肠）。LPS攻击持续降低了MCT6/8组和AGP组的绒毛高度和隐窝深度。然而，补充MCT6/8的LPS攻击仔猪恢复了绒毛高度，而AGP组没有。MCT6/8组仔猪的血清IgA、空肠IgA阳性浆细胞和杯状细胞比AGP组更多。在回肠水平，结果相似，但不太明显。本研究为断奶后时期MCT6/8优于AGP的益处提供了新的见解。C6和C8对大肠杆菌具有体外抗菌作用。在体内，MCT6/8对小肠也有保护作用，可能促进生长。
