大鼠睾丸血管紧张素I转换酶：纯化及其与大鼠肺酶的比较。

Rat testicular angiotensin I converting enzyme: purification and comparison with rat pulmonary enzyme.

作者信息

Yamaguchi T, Hiratsuka M, Ikekita M, Kizuki K, Moriya H

出版信息

Adv Exp Med Biol. 1986;198 Pt A:461-7. doi: 10.1007/978-1-4684-5143-6_62.

Abstract

Enzymological properties of rat testicular angiotensin I converting enzyme (RT-ACE) were compared with those of rat pulmonary angiotensin I converting enzyme (RP-ACE). The molecule of RT-ACE was different from that of RP-ACE with respect to the molecular weight, i.e., the molecular weight of RT-ACE was estimated to be 104 kilo-dalton (kd) and that of RP-ACE (150 kd) on SDS-polyacrylamide gel electrophoresis. On the other hand, the enzymochemical properties of RT-ACE were very similar to those of RP-ACE, with regard to activation by NaCl, optimum pH, Km value for N*-hippuryl-His-Leu-OH hydrolysis and sensitivities to various inhibitors. Therefore, it was speculated that the portions contributing to the appearance of catalytic activity would be similar between RT-ACE and RP-ACE.

摘要

将大鼠睾丸血管紧张素I转换酶（RT-ACE）的酶学特性与大鼠肺血管紧张素I转换酶（RP-ACE）的酶学特性进行了比较。RT-ACE的分子在分子量方面与RP-ACE不同，即在SDS-聚丙烯酰胺凝胶电泳上，RT-ACE的分子量估计为104千道尔顿（kd），而RP-ACE的分子量为150 kd。另一方面，就NaCl激活、最适pH、N*-马尿酰-组氨酸-亮氨酸-OH水解的Km值以及对各种抑制剂的敏感性而言，RT-ACE的酶化学特性与RP-ACE非常相似。因此，推测RT-ACE和RP-ACE之间对催化活性表现有贡献的部分是相似的。