Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK.
Nat Prod Rep. 2018 Oct 17;35(10):1097-1109. doi: 10.1039/c8np00066b.
Covering: up to 2018 The construction of polyketide natural products by type I modular polyketide synthases (PKSs) requires the coordinated action of several protein subunits to ensure biosynthetic fidelity. This is particularly the case for trans-AT PKSs, which in contrast to most cis-AT PKSs, contain split modules and employ several trans-acting catalytic domains. This article summarises recent advances in understanding the protein-protein interactions underpinning subunit assembly and intra-subunit communication in such systems and highlights potential avenues and approaches for future research.
截至 2018 年 Ⅰ 型模块化聚酮合酶（PKSs）构建聚酮天然产物需要几种蛋白质亚基的协调作用，以确保生物合成的保真度。对于反式 AT PKS 尤其如此，与大多数顺式 AT PKS 相比，反式 AT PKS 包含分裂模块，并采用几种反式作用催化结构域。本文总结了近年来在理解这些系统中亚基组装和亚基内通讯所必需的蛋白-蛋白相互作用方面的最新进展，并强调了未来研究的潜在途径和方法。
