EICU, Yantai Yuhuangding Hospital, Yantai, China.
Eur Rev Med Pharmacol Sci. 2018 Sep;22(18):6149-6155. doi: 10.26355/eurrev_201809_15955.
To study the protective effect of simvastatin on arterial plaque instability induced by p-cresyl sulfate (PCS).
Apolipoprotein E (ApoE)-/- mice were selected as objects of this study. All mice were randomly divided into three groups: 1) the control group, 2) the PCS group and 3) the PCS + simvastatin group. After successful modeling, the levels of plasma cholesterol, triglyceride, low-density lipoprotein, high-density lipoprotein, interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-β (TNF-β) were detected. The gross specimen of coronary artery was stained. Meanwhile, oil red O staining and Sirius red staining were performed for coronary arterial sections to observe the lipid and collagen components. The expression levels of smooth muscle cells and macrophages were observed by immunohistochemistry. In addition, the expression levels of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs) and monocyte chemoattractant protein-1 (MCP-1) in tissues were detected by Western blotting.
Simvastatin could improve atherosclerotic plaque growth and atherosclerotic plaque instability induced by PCS. Moreover, simvastatin could also improve the changes of MMPs and TIMPs caused by PCS as well as the inflammatory status in mice.
Simvastatin can improve the inflammatory status in mice, eventually improving the arterial plaque instability caused by PCS.
研究辛伐他汀对 p- 硫酸对甲酚（PCS）诱导的动脉斑块不稳定性的保护作用。
选择载脂蛋白 E（ApoE）-/- 小鼠作为本研究的对象。所有小鼠随机分为三组：1）对照组，2）PCS 组和 3）PCS+辛伐他汀组。成功建模后，检测血浆胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白、白细胞介素-1（IL-1）、白细胞介素-6（IL-6）和肿瘤坏死因子-β（TNF-β）水平。对冠状动脉的大体标本进行染色。同时，对冠状动脉切片进行油红 O 染色和天狼星红染色，观察脂质和胶原成分。通过免疫组织化学观察平滑肌细胞和巨噬细胞的表达水平。此外，通过 Western blot 检测组织中基质金属蛋白酶（MMPs）、金属蛋白酶组织抑制剂（TIMPs）和单核细胞趋化蛋白-1（MCP-1）的表达水平。
辛伐他汀可以改善 PCS 诱导的动脉粥样硬化斑块生长和动脉粥样硬化斑块不稳定性。此外，辛伐他汀还可以改善 PCS 引起的 MMPs 和 TIMPs 的变化以及小鼠的炎症状态。
辛伐他汀可以改善小鼠的炎症状态，最终改善 PCS 引起的动脉斑块不稳定性。
