School of Mechanical Engineering, Hebei University of Science and Technology, Shijiazhuang, 050018, China; Department of Dermatology, School of Medicine, University of California, San Francisco, CA, 94143, USA.
Department of Dermatology, School of Medicine, University of California, San Francisco, CA, 94143, USA.
Toxicol Lett. 2018 Dec 15;299:118-123. doi: 10.1016/j.toxlet.2018.09.013. Epub 2018 Sep 30.
This study compared the efficiency for in vitro human skin decontamination using DDGel and RSDL when applied at different timepoints (5 min and 90 min). Experiments were performed using in vitro human skin models, in which skin was mounted onto Flow-Through diffusion cells. The mass of -C DIMP removed from skin surface after decontamination was quantitated by measuring radioactivity with a liquid scintillation spectrometer. Both decontaminants removed more than 90% recovery dose of DIMP from skin compared to control group (p < 0.05). DDGel skin decontamination reduced more toxicant amount when compared to RSDL. DDGel showed slight higher decontamination ability of DIMP than RSDL and efficiently removed chemicals from the skin surface, also reduced the amount of DIMP in receptor fluid. A similar decontamination regimen with RSDL and DDGel at 90 min showed that both were still might effectively increase the time window of opportunity for treatment. Thus, DDGel can offer a potential as a next generation skin decontamination platform technology for military and civilian applications.
本研究比较了在不同时间点（5 分钟和 90 分钟）应用时，DDGel 和 RSDL 对体外人体皮肤去污的效率。实验采用体外人体皮肤模型进行，其中皮肤安装在流动式扩散细胞上。用液体闪烁光谱仪测量放射性来定量测定去污后从皮肤表面去除的 -C DIMP 的质量。与对照组相比（p<0.05），两种去污剂都从皮肤中去除了超过 90%的 DIMP 回收剂量。与 RSDL 相比，DDGel 皮肤去污时减少了更多的有毒物质。与 RSDL 相比，DDGel 对 DIMP 的去污能力略高，能有效地从皮肤表面去除化学物质，也减少了受体液中的 DIMP 含量。在 90 分钟时，使用 RSDL 和 DDGel 进行类似的去污方案表明，两者仍然可以有效地增加治疗的机会时间窗口。因此，DDGel 可以作为军事和民用应用的下一代皮肤去污平台技术提供潜力。
