Xu Wenting, Miyamoto Licht, Aihara Haruna, Yamaoka Tomomi, Tanaka Naonobu, Tsuchihashi Yuki, Ikeda Yasumasa, Tamaki Toshiaki, Kashiwada Yoshiki, Tsuchiya Koichiro
Department of Medical Pharmacology, Institute of Biomedical Sciences, Graduate School of Tokushima University.
Department of Pharmacology, Institute of Biomedical Sciences, Graduate School of Tokushima University.
J Med Invest. 2018;65(3.4):225-230. doi: 10.2152/jmi.65.225.
Ectopic fat accumulation is associated with insulin resistance and type 2 diabetes mellitus. Citrus sudachi is an evergreen tree that is found mainly in Tokushima Prefecture in Japan. Previously, it was demonstrated that Citrus sudachi could inhibit the rising trend of blood glucose and fatty acid in human subjects. In the current study, we illustrated the function of methanol extracts from sudachi peel and investigated the mechanism of this effect. We got the five kinds of methanol extracts by using diaion HP-20, and those were named by hydrophobicity from M-F1 to M-F5. Among the 5 kinds of sudachi methanol extracts, only M-F4 significantly decreased the intracellular triglyceride of C2C12 cells. It augmented the AMPK activity and increased the transcription of PPARα and its downstream targets CPT-1b and UCP2. In conclusion, M-F4 improved the lipid metabolism possibly through AMPK, PPARα and their downstream targets like CPT-1b and UCP2. Furthermore, this extract may be useful for preventing obesity and diabetes related diseases. J. Med. Invest. 65:225-230, August, 2018.
异位脂肪堆积与胰岛素抵抗和2型糖尿病有关。德岛酸橙是一种主要生长在日本德岛县的常绿树。此前有研究表明，德岛酸橙可以抑制人体血糖和脂肪酸的上升趋势。在本研究中，我们阐述了德岛酸橙果皮甲醇提取物的功能，并研究了其作用机制。我们使用Diaion HP-20获得了五种甲醇提取物，并根据疏水性将它们命名为M-F1至M-F5。在这5种德岛酸橙甲醇提取物中，只有M-F4能显著降低C2C12细胞内的甘油三酯。它增强了AMPK活性，并增加了PPARα及其下游靶点CPT-1b和UCP2的转录。总之，M-F4可能通过AMPK、PPARα及其下游靶点如CPT-1b和UCP2改善脂质代谢。此外，这种提取物可能对预防肥胖和糖尿病相关疾病有用。《医学调查杂志》65:225 - 230，2018年8月。
