γ射线对DNA与核内非组蛋白相互作用的影响。
The effect of gamma-radiation on the interaction of DNA with nuclear non-histone proteins.
作者信息
Umansky S R, Domashenko A D
出版信息
Int J Radiat Biol Relat Stud Phys Chem Med. 1977 Oct;32(4):361-7. doi: 10.1080/09553007714551111.
The interaction of nuclear sap proteins and chromatin non-histone proteins with DNA was studied by two methods: membrane filter technique and chromatography of 32P-labelled proteins on DNA-containing columns. Irradiated DNA binds a slightly greater amount of these proteins and much more firmly than the native DNA. The effect is caused by the appearance of the locally denatured sites in irradiated DNA. Irradiation of non-histone proteins disturbs their specific interaction with DNA more easily than the non-specific binding.
采用两种方法研究了核液蛋白和染色质非组蛋白与DNA的相互作用：膜过滤技术和含DNA柱上32P标记蛋白的色谱分析。与天然DNA相比，辐照后的DNA结合的这些蛋白量略多且结合更牢固。这种效应是由辐照后DNA中局部变性位点的出现引起的。与非特异性结合相比，辐照非组蛋白更容易干扰它们与DNA的特异性相互作用。
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