The effect of gamma-radiation on the interaction of DNA with nuclear non-histone proteins.

The interaction of nuclear sap proteins and chromatin non-histone proteins with DNA was studied by two methods: membrane filter technique and chromatography of 32P-labelled proteins on DNA-containing columns. Irradiated DNA binds a slightly greater amount of these proteins and much more firmly than the native DNA. The effect is caused by the appearance of the locally denatured sites in irradiated DNA. Irradiation of non-histone proteins disturbs their specific interaction with DNA more easily than the non-specific binding.