[γ射线照射大鼠胸腺和肝脏中与DNA结合的非组蛋白的组成变化]
[Changes in the composition of DNA-bound nonhistone protein in the thymus and liver of gamma-irradiated rats].
作者信息
Strazhevskaia N B, Struchkov V A, Blokhin D Iu
出版信息
Radiobiologiia. 1989 Jul-Aug;29(4):441-4.
PMID:2780976
Abstract
A supramolecular DNA complex (SC DNA) and DNA of a phenol nuclear matrix (PNM DNA) were extracted, by the phenol method, from rat thymus and liver 15 min following 10 Gy gamma-irradiation. The method of electrophoresis in polyacrylamide gel was used to study a composition of nonhistone proteins firmly bound to these DNA fractions. Irradiation was shown to induce the occurrence of new proteins and redistribution of proteins between SC DNA and PNN DNA of rat organs.
摘要
采用苯酚法从经10 Gy γ射线照射15分钟后的大鼠胸腺和肝脏中提取超分子DNA复合物（SC DNA）和酚核基质DNA（PNM DNA）。运用聚丙烯酰胺凝胶电泳法研究与这些DNA组分紧密结合的非组蛋白的组成。结果表明，辐射可诱导大鼠器官的SC DNA和PNN DNA中出现新蛋白质并导致蛋白质重新分布。
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