Laaksonen A M, Mäntyjärvi R A, Hänninen O O
Arch Toxicol Suppl. 1986;9:247-50. doi: 10.1007/978-3-642-71248-7_36.
Secondary cultures of newborn NMRI nu/nu (nude) mouse skin fibroblasts were used as targets for transformation by the combined administration of SV40 and 3-methylcholanthrene (MC). The long (72 h) post-treatment with MC increased virus transformation as much as 4.3-fold. In contrast, anthracene, a non-carcinogenic compound, had no effect on viral transformation frequency. Despite considerable variation within a group, the cell lines transformed by the combination treatment, as a group, were more tumourigenic than cell lines transformed by SV40 alone.
新生NMRI裸鼠皮肤成纤维细胞的传代培养物被用作SV40和3-甲基胆蒽（MC）联合处理的转化靶标。MC处理72小时后，病毒转化增加了4.3倍。相比之下，蒽这种非致癌化合物对病毒转化频率没有影响。尽管组内存在相当大的差异，但联合处理转化的细胞系作为一个整体，比单独用SV40转化的细胞系更具致瘤性。
