The A.O. Kovalevsky Institute of Marine Biology Research of RAS, Nakhimov av., 2, Sevastopol, Russia, 299011.
Department of Medicine, Novosibirsk State University, Pirogova st., 1, Novosibirsk, Russia, 630090.
J Mol Evol. 2018 Oct;86(8):566-580. doi: 10.1007/s00239-018-9868-2. Epub 2018 Oct 3.
Transposable elements represent the DNA fragments capable of increasing their copy number and moving within the genome. Class II mobile elements represents the DNA transposons, which transpose via excision and the subsequent reinsertion at random genomic loci. The increase of their copy number occurs only when the transposition event is coupled with the replication. IS630/Tc1/mariner DNA transposon superfamily is one of the largest and widely distributed among the Class II elements. In this work, we provide a detailed analysis of IS630/Tc1/mariner DNA transposons from the Pacific oyster, Crassostrea gigas. IS630/Tc1/mariner transposons represented in the genome of the Pacific oyster belong to four families, Tc1 (DD34E), mariner (DD34D), pogo (DDxD), and rosa (DD41D). More than a half of IS630/Tc1/mariner elements from C. gigas belong to Tc1 family. Furthermore, Mariner-31_CGi element was shown to represent a new and previously unknown family with DD37E signature. We also discovered the full-size transcripts of eight elements from Tc1, mariner, and pogo families, three of which can, presumably, retain their transposition activity.
转座元件是指能够增加其拷贝数并在基因组内移动的 DNA 片段。第二类移动元件代表 DNA 转座子，它们通过切除和随后在随机基因组位点的重新插入进行转座。只有在转座事件与复制偶联时，它们的拷贝数才会增加。IS630/Tc1/mariner DNA 转座子超家族是第二类元件中最大和分布最广的家族之一。在这项工作中，我们对太平洋牡蛎（Crassostrea gigas）中的 IS630/Tc1/mariner DNA 转座子进行了详细分析。IS630/Tc1/mariner 转座子在太平洋牡蛎基因组中的代表属于四个家族，即 Tc1（DD34E）、mariner（DD34D）、pogo（DDxD）和 rosa（DD41D）。超过一半的太平洋牡蛎 IS630/Tc1/mariner 元件属于 Tc1 家族。此外，Mariner-31_CGi 元件代表了一个具有 DD37E 特征的新的、以前未知的家族。我们还发现了来自 Tc1、mariner 和 pogo 家族的八个元件的全长转录本，其中三个可能保留了它们的转座活性。
