Oh Dool-Ri, Kim Yujin, Choi Eun-Jin, Jo Ara, Shin Jawon, Kang Huwon, Lee Seul-Gi, Kim Jaeyong, Kim Young Ran, Choi Chul Yung
* Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research (JINR), Jeollanamdo 59338, Republic of Korea.
† College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 61186, Republic of Korea.
Am J Chin Med. 2018 Oct 4:1-20. doi: 10.1142/S0192415X18500775.
The present study evaluates the anti-oxidative stress activity of Vaccinium bracteatum Thunb. fruit extract (VBFW) to identify the mechanisms responsible for its antidepressant-like effects. To evaluate the antidepressant and anti-oxidant effects of VBFW, malondialdehyde (MDA), serotonin transporter (SERT), and monoamine oxidase A (MAO-A) levels were measured in a mouse model of chronic restraint stress (CRS). The underlying mechanisms preventing oxidative stress and neuronal apoptosis were investigated using in vitro models of hydrogen peroxide (HO[Formula: see text]-induced neuronal damage. The results showed that VBFW treatment (200[Formula: see text]mg/kg) significantly reduced MDA, SERT, and MAO-A levels in the prefrontal cortex of CRS mice. Furthermore, VBFW (30[Formula: see text][Formula: see text]g/mL) exhibited protective effects against HO-induced cell death via inhibition of the HO-induced increase in Bax and decrease in Bcl-2 levels within the mitochondria of SH-SY5Y cells. Furthermore, VBFW (10 and 30[Formula: see text][Formula: see text]g/mL) exerted protective effects against HO-induced cell death through inhibition of key mitochondria-associated apoptotic proteins such as cytochrome c, caspase-3 and PARP. Additionally, VBFW (10 and 30[Formula: see text][Formula: see text]g/mL) could improve the activity of anti-oxidant enzymes (such as SOD and catalase) in HO-treated SH-SY5Y cells. These results suggest that the antidepressant and anti-oxidant effects of VBFW might be mediated by the regulation of SERT and MAO-A, and possibly associated with regulation of oxidative stress-induced apoptosis.
本研究评估了乌饭树果实提取物（VBFW）的抗氧化应激活性，以确定其抗抑郁样作用的机制。为了评估VBFW的抗抑郁和抗氧化作用，在慢性束缚应激（CRS）小鼠模型中测量了丙二醛（MDA）、5-羟色胺转运体（SERT）和单胺氧化酶A（MAO-A）的水平。使用过氧化氢（HO[公式：见正文]）诱导的神经元损伤体外模型研究了预防氧化应激和神经元凋亡的潜在机制。结果表明，VBFW处理（200[公式：见正文]mg/kg）显著降低了CRS小鼠前额叶皮质中的MDA、SERT和MAO-A水平。此外，VBFW（30[公式：见正文][公式：见正文]g/mL）通过抑制HO诱导的SH-SY5Y细胞线粒体内Bax增加和Bcl-2水平降低，对HO诱导的细胞死亡具有保护作用。此外，VBFW（10和30[公式：见正文][公式：见正文]g/mL）通过抑制关键的线粒体相关凋亡蛋白如细胞色素c、半胱天冬酶-3和聚（ADP-核糖）聚合酶，对HO诱导的细胞死亡发挥保护作用。此外，VBFW（10和30[公式：见正文][公式：见正文]g/mL）可以提高HO处理的SH-SY5Y细胞中抗氧化酶（如超氧化物歧化酶和过氧化氢酶）的活性。这些结果表明，VBFW的抗抑郁和抗氧化作用可能是通过对SERT和MAO-A的调节介导的，并且可能与氧化应激诱导的细胞凋亡调节有关。
