1 The Research Institute at Nationwide Children's Hospital, Columbus, Ohio.
2 Nanofiber Solutions, Hilliard, Ohio.
Tissue Eng Part A. 2019 Jun;25(11-12):878-886. doi: 10.1089/ten.TEA.2018.0226. Epub 2018 Nov 7.
This study compared side-by-side the impact of donor age on the production of tissue-engineered small intestine (TESI). Each age represents a specific period of life: E18 for fetuses, 5-day-old pups for neonates, 21-day-old rats for weanlings, and 6-week-old rats for adults. The TESI produced was compared macroscopically and microscopically. The mechanism(s) contributing to the differences observed was explored by detecting proliferating cells in the TESI and by analyzing intestinal stem cell gene expression in donor cells. These data may provide valuable information for future application of TESI clinically.
本研究并列比较了供体年龄对组织工程小肠(TESI)生成的影响。每个年龄代表生命的一个特定时期:E18 代表胎儿,5 天大的幼崽代表新生儿,21 天大的幼鼠代表断奶期,6 周龄的大鼠代表成年期。比较了生成的 TESI 的宏观和微观特征。通过检测 TESI 中的增殖细胞,并分析供体细胞中肠干细胞基因表达,探索了导致观察到的差异的机制。这些数据可为 TESI 的临床应用提供有价值的信息。