五味子通过下调 NFATc1 和 c-fos 表达抑制 RANKL 刺激的破骨细胞分化。
Inhibition of RANKL-stimulated osteoclast differentiation by Schisandra chinensis through down-regulation of NFATc1 and c-fos expression.
机构信息
College of Korean Medicine, Dongguk University, Gyeongju, Republic of Korea.
Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.
出版信息
BMC Complement Altern Med. 2018 Oct 1;18(1):270. doi: 10.1186/s12906-018-2331-5.
BACKGROUND
Schisandra chinenesis (SC) has been reported to have ameliorative effect on osteoporosis. However, the mechanisms underlying the anti-osteoporosis activity of SC have not been clearly elucidated. In the present study, we determined the effects of SC on The receptor activator of NF-kB ligand (RANKL)-induced osteoclastogenesis and its potential mechanism.
METHODS
Raw 264.7 cells were treated with 0.6, 6 and 60 μg/mL SC in the presence of 100 ng/mL RANKL for 7 days. RANKL-induced osteoclast formation was analyzed by tartrate resistant acid phosphatase (TRAP) staining. The osteoclast differentiation-related factors were confirmed along with TNF-α.
RESULTS
SC inhibits the RANKL-induced osteoclast differentiation in dose-dependent manner within non-toxic concentrations. The supernatant concentrations of TNF-α were significantly decreased by SC treatment. In addition, osteoclastogenesis-related factors, TRAP6 and NF-κB, were markedly decreased by SC in RANKL-induced osteoclasts. Mechanistically, SC reduced the RANKL-triggered NFATc1 and c-fos expressions.
CONCLUSIONS
Taken together, our data suggest that SC can modulate bone metabolism by suppressing RANKL-induced osteoclast differentiation.
背景
五味子已被报道具有改善骨质疏松的作用。然而，五味子抗骨质疏松活性的机制尚未阐明。在本研究中，我们测定了五味子对核因子-κB 受体激活剂配体（RANKL）诱导的破骨细胞分化的作用及其潜在机制。
方法
在存在 100ng/ml RANKL 的情况下，用 0.6、6 和 60μg/ml 的五味子处理 Raw 264.7 细胞 7 天。用抗酒石酸酸性磷酸酶（TRAP）染色分析 RANKL 诱导的破骨细胞形成。同时，与 TNF-α 一起确认破骨细胞分化相关因子。
结果
五味子在无毒浓度范围内以剂量依赖性方式抑制 RANKL 诱导的破骨细胞分化。SC 处理可显著降低上清液中 TNF-α 的浓度。此外，SC 还可显著降低 RANKL 诱导的破骨细胞中破骨细胞分化相关因子 TRAP6 和 NF-κB 的表达。
结论
综上所述，我们的数据表明，五味子可以通过抑制 RANKL 诱导的破骨细胞分化来调节骨代谢。
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