Oh Su-Yeon, Kim Young Hoon, Bae Deok Sung, Um Byung Hun, Pan Cheol-Ho, Kim Chul Young, Lee Hee Ju, Lee Jae Kwon
Department of Biology Education, College of Education, Chungbuk National University, Chungcheongbuk-Do, Republic of Korea.
Biosci Biotechnol Biochem. 2010;74(2):285-91. doi: 10.1271/bbb.90597. Epub 2010 Feb 7.
Schiandra chinensis is a well-known Chinese traditional medicine for the treatment of hepatic disease. In this study, we investigated whether the nine major compounds of Schiandra chinensis could be applied to suppress lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (Raw 264.7 cells). Among the nine lignans, three, gomisin J, gomisin N, and schisandrin C, were found to reduce nitric oxide (NO) production from LPS-stimulated Raw 264.7 cells. These three lignans showed low cytotoxic effects in Raw 264.7 cells. Pre-treatment of Raw 264.7 cells with gomisin J, gomisin N, or schisandrin C reduced the expression of mRNA and the secretion of pro-inflammatory cytokines. These inhibitory effects were found to be caused by blockage of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinases 1 and 2 (ERK 1/2), and c-Jun N-terminal kinase (JNK) phosphorylation.
五味子是一种治疗肝病的著名中药。在本研究中，我们研究了五味子的九种主要化合物是否可用于抑制脂多糖（LPS）诱导的小鼠巨噬细胞（Raw 264.7细胞）炎症反应。在这九种木脂素中，发现三种，即戈米辛J、戈米辛N和五味子醇甲，可减少LPS刺激的Raw 264.7细胞中一氧化氮（NO）的产生。这三种木脂素在Raw 264.7细胞中显示出低细胞毒性作用。用戈米辛J、戈米辛N或五味子醇甲预处理Raw 264.7细胞可降低mRNA表达和促炎细胞因子的分泌。发现这些抑制作用是由p38丝裂原活化蛋白激酶（MAPK）、细胞外信号调节激酶1和2（ERK 1/2）以及c-Jun N端激酶（JNK）磷酸化的阻断引起的。
