Leftwich J A, Carlson P, Adelman B, Hall R E
Cancer Res. 1987 Mar 1;47(5):1319-24.
The HL-60 (human promyelocytic leukemia) cell line has proved to be a useful model for the study of the expression of cellular functions and markers associated with hematopoietic differentiation. We report here the development and initial characterization of a novel, differentiation-resistant HL-60 cell line (HL-60-1E3) which was established by cloning the parent HL-60 line in the absence of mutagens or differentiation-inducing agents. HL-60-1E3 exhibits markedly reduced phorbol diester-induced expression of extracellular cytolytic activity, nonspecific esterase, phagocytosis, and surface Mo1 antigen. In addition, dimethyl sulfoxide-induced expression of both Mo1 and Mo2 is markedly reduced. However, if HL-60-1E3 is exposed to dimethyl sulfoxide, it acquires appreciable phorbol diester-triggered cytolytic activity and production of superoxide anion (O2-). Phorbol diester receptor number and dissociation constant (Kd) obtained by Scatchard analysis are not significantly different for the two cell lines. The HL-60-1E3 cell line should provide a useful adjunct to other cell lines used in the study of normal myeloid and leukemic cell differentiation, as well as the study of cytokine maturation factors and oncogene expression.
HL-60（人早幼粒细胞白血病）细胞系已被证明是研究与造血分化相关的细胞功能和标志物表达的有用模型。我们在此报告一种新型的、抗分化的HL-60细胞系（HL-60-1E3）的建立及其初步特征，该细胞系是通过在无诱变剂或分化诱导剂的情况下克隆亲代HL-60细胞系而建立的。HL-60-1E3表现出佛波酯诱导的细胞外溶细胞活性、非特异性酯酶、吞噬作用和表面Mo1抗原的表达明显降低。此外，二甲基亚砜诱导的Mo1和Mo2的表达也明显降低。然而，如果HL-60-1E3暴露于二甲基亚砜，它会获得明显的佛波酯触发的溶细胞活性和超氧阴离子（O2-）的产生。通过Scatchard分析获得的佛波酯受体数量和解离常数（Kd）在两种细胞系中没有显著差异。HL-60-1E3细胞系应为用于研究正常髓系和白血病细胞分化以及细胞因子成熟因子和癌基因表达的其他细胞系提供有用的辅助。
