Hawrylak K, Stinson R A
FEBS Lett. 1987 Feb 23;212(2):289-91. doi: 10.1016/0014-5793(87)81362-5.
Membrane-bound human liver alkaline phosphatase solubilized by a non-ionic detergent, Nonidet P-40 (NP-40), has the molecular mass of a tetramer. It can be converted to a dimeric form by treatment with a phosphatidylinositol phospholipase C (PI-PLC) obtained from Bacillus cereus. When human liver plasma membranes were directly treated with PI-PLC, the released alkaline phosphatase was dimeric. Thus, phosphatidylinositol may help maintain the tetrameric quaternary structure of alkaline phosphatase and aid its binding to human liver plasma membranes.
用非离子去污剂Nonidet P - 40（NP - 40）增溶的膜结合型人肝碱性磷酸酶具有四聚体的分子量。用蜡状芽孢杆菌来源的磷脂酰肌醇磷脂酶C（PI - PLC）处理后，它可转变为二聚体形式。当用人肝质膜直接用PI - PLC处理时，释放出的碱性磷酸酶是二聚体。因此，磷脂酰肌醇可能有助于维持碱性磷酸酶的四聚体四级结构，并有助于其与人肝质膜的结合。
