Kishikawa H, Takeda H, Kiyota S, Sakakida M, Fukushima H, Ichinose K, Matsuda H, Nakamura N, Uzawa H
Diabetes. 1987 Apr;36(4):407-12. doi: 10.2337/diab.36.4.407.
The effects of alpha 2-adrenergic-receptor blocker mianserin on the responses of blood glucose, plasma beta-thromboglobulin (beta-TG), and various counterregulatory hormones to insulin-induced hypoglycemia were studied in nine healthy male subjects. The alpha 2-adrenoceptor-blocking action of mianserin was confirmed by its inhibitory effect on platelet activation in vitro. Mianserin was given orally 90 min before insulin injection; the same study without mianserin was performed on another day as the control study. The time courses of blood glucose and serum C-peptide (0, 20, 45, and 180 min after the insulin injection) were identical in both studies, indicating that mianserin has no effect on these parameters. However, a significant increase of beta-TG at 45 min after insulin injection was completely suppressed by the administration of mianserin (mean +/- SE, 68.5 +/- 6.0 vs. 28.8 +/- 7.6 ng/ml, n = 6, P less than .05). No significant differences were obtained between the two studies in the responses of plasma or serum catecholamines, cortisol, glucagon, growth hormone, thromboxane B2, and 6-ketoprostaglandin F1 alpha. These results suggest that epinephrine is responsible for some, if not all, of the beta-TG release from the platelets during insulin-induced hypoglycemia.
在9名健康男性受试者中研究了α2-肾上腺素能受体阻滞剂米安色林对血糖、血浆β-血小板球蛋白（β-TG）以及各种反调节激素对胰岛素诱导低血糖反应的影响。米安色林对体外血小板活化的抑制作用证实了其α2-肾上腺素能受体阻断作用。在注射胰岛素前90分钟口服米安色林；在另一天进行不使用米安色林的相同研究作为对照研究。两项研究中血糖和血清C肽的时间进程（胰岛素注射后0、20、45和180分钟）相同，表明米安色林对这些参数无影响。然而，胰岛素注射后45分钟时β-TG的显著升高被米安色林给药完全抑制（均值±标准误，68.5±6.0对28.8±7.6 ng/ml，n = 6，P<0.05）。两项研究在血浆或血清儿茶酚胺、皮质醇、胰高血糖素、生长激素、血栓素B2和6-酮前列腺素F1α的反应方面未获得显著差异。这些结果表明，在胰岛素诱导的低血糖期间，肾上腺素即使不是全部，也是部分导致血小板释放β-TG的原因。
