Clinical Eye Research Division, Department of Ophthalmology, Zealand University Hospital, Roskilde, Denmark.
Faculty of Health and Medical Science, University of Copenhagen, Copenhagen, Denmark.
Acta Ophthalmol. 2019 Feb;97(1):99-106. doi: 10.1111/aos.13886. Epub 2018 Oct 4.
Ageing is the strongest predictor of neovascular age-related macular degeneration (AMD), where neuroinflammation is known to play a major role. Less is known about polypoidal choroidal vasculopathy (PCV), which is an important differential diagnosis to neovascular AMD. Here, we report plasma markers of inflammation with age (inflammaging) in patients with PCV, patients with neovascular AMD and a healthy age-matched control group.
We isolated plasma from fresh venous blood obtained from participants (n = 90) with either PCV, neovascular AMD, or healthy maculae. Interleukin(IL)-1β, IL-6, IL-8, IL-10 and tumour necrosis factor receptor 2 (TNF-R2) were measured using U-PLEX Human Assays. Routine plasma C-reactive protein (CRP) was measured using Dimension Vista 1500.
Patients with PCV had plasma levels of IL-1β, IL-6, IL-8, IL-10 and TNF-R2 similar to that in healthy controls. Patients with neovascular AMD had significantly higher plasma IL-1β, IL-6 and IL-10 than healthy controls, whereas no significant differences were observed for plasma IL-8 and TNF-R2. Differences between plasma IL-1β, IL-6 and IL-10 possessed a positive but weak ability in discriminating neovascular AMD from PCV. Both patients with PCV and patients with neovascular AMD had significantly higher levels of routine plasma CRP.
Patients with PCV differ from patients with neovascular AMD in terms of plasma inflammaging profile. Apart from increased CRP, no signs of inflammaging were observed in patients with PCV. In patients with neovascular AMD, we find a specific angiogenesis-twisted inflammaging profile.
衰老（aging）是年龄相关性黄斑变性（AMD）中新生血管形成的最强预测因子，已知神经炎症（neuroinflammation）在此过程中发挥重要作用。关于息肉样脉络膜血管病变（PCV），其是新生血管性 AMD 的重要鉴别诊断，人们了解较少。在此，我们报告了 PCV 患者、新生血管性 AMD 患者和健康年龄匹配对照组中与年龄相关的炎症标志物（inflammaging）。
我们从 PCV、新生血管性 AMD 或健康黄斑患者的新鲜静脉血中分离血浆。使用 U-PLEX 人分析物测量白细胞介素（IL）-1β、IL-6、IL-8、IL-10 和肿瘤坏死因子受体 2（TNF-R2）。使用 Dimension Vista 1500 测量常规血浆 C 反应蛋白（CRP）。
PCV 患者的血浆 IL-1β、IL-6、IL-8、IL-10 和 TNF-R2 水平与健康对照组相似。新生血管性 AMD 患者的血浆 IL-1β、IL-6 和 IL-10 水平明显高于健康对照组，而 IL-8 和 TNF-R2 则无显著差异。区分新生血管性 AMD 和 PCV 的血浆 IL-1β、IL-6 和 IL-10 差异具有正相关但较弱的能力。PCV 患者和新生血管性 AMD 患者的常规血浆 CRP 水平均显著升高。
PCV 患者与新生血管性 AMD 患者的血浆炎症标志物谱不同。除了 CRP 增加外，PCV 患者未观察到炎症迹象。在新生血管性 AMD 患者中，我们发现了一种特定的血管生成扭曲的炎症标志物谱。
