Department of Wine and Food Science , The University of Adelaide (UA) , PMB 1 , Glen Osmond , South Australia 5064 , Australia.
The Australian Wine Research Institute (AWRI) , P.O. Box 197, Glen Osmond , South Australia 5064 , Australia.
J Agric Food Chem. 2018 Oct 17;66(41):10808-10815. doi: 10.1021/acs.jafc.8b04027. Epub 2018 Oct 5.
On the basis of the chemistry of wine and the co-occurrence of 3-sulfanylhexan-1-ol (3-SH) and acetaldehyde, we investigated the existence of 2-methyl-4-propyl-1,3-oxathiane (1) and identified the presence of a single detectable geometric isomer, cis-1, in wines for the first time. A stable isotope dilution assay (SIDA) using headspace-solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) was developed and validated, and used to quantitate cis-1 in a survey of wines, revealing a range from undetectable (limit of detection = 2.6 ng/L) to 460 ng/L. The odor detection threshold of 1 (using a standard comprising 85% cis-1 and 15% trans-1) in neutral white wine was determined to be 7.1 μg/L. Despite cis-1 not appearing above the determined sensory threshold in the studied wines, the findings demonstrated the presence of a new volatile sulfur compound with a strong correlation to 3-SH concentration ( r = 0.72), showing that cis-1 has potential implications for the fate of the important wine aroma compound 3-SH.
基于葡萄酒的化学性质以及 3-巯基己烷-1-醇（3-SH）和乙醛的共同存在，我们首次研究了 2-甲基-4-丙基-1,3-氧杂环戊烷（1）的存在，并确定了其存在单一可检测的顺式异构体 cis-1。建立并验证了一种使用顶空固相微萃取（HS-SPME）和气相色谱-质谱（GC-MS）的稳定同位素稀释分析（SIDA）方法，并用于定量检测葡萄酒中的 cis-1，结果显示其浓度范围为不可检测（检测限=2.6 ng/L）至 460 ng/L。在中性白葡萄酒中，1 的气味检测阈值（使用包含 85% cis-1 和 15% trans-1 的标准品）被确定为 7.1 μg/L。尽管在研究的葡萄酒中 cis-1 并未超过感官阈值，但这些发现表明存在一种新的挥发性硫化合物，与 3-SH 浓度具有很强的相关性（r=0.72），表明 cis-1 可能对重要葡萄酒香气化合物 3-SH 的命运产生影响。
