Ariga H, Naruto M, Kawano H, Nakano H
J Biochem. 1986 Oct;100(4):1103-6. doi: 10.1093/oxfordjournals.jbchem.a121792.
Of the several DNA fragments present in the human lung cancer gene, 1.1 and 2.0 kilobase (kb) fragments corresponding to the intron of this gene were hybridized to a half part of the 27 nucleotides perfect palindrome present in the initiation part of replication in simian virus 40 (SV40) DNA. These two fragments cloned in pBR322 had good template activity, and the initiation of DNA replication started from the region of these fragments in an in vitro system, in which the initiation of DNA replication occurs on cloned DNA containing SV40 origin of DNA replication as described previously. Furthermore, these two clones could replicate autonomously in nuclei of SV40 transformed Cos cells, producing SV40 T antigen constitutively when the clones were transfected into Cos cells. These results show that functional SV40 origin-like sequences are present in human genomes, and they can replicate autonomously within the cells which are producing SV40 T antigen.
在人类肺癌基因中存在的几个DNA片段中，与该基因内含子相对应的1.1和2.0千碱基（kb）片段，与猴病毒40（SV40）DNA复制起始部分存在的27个核苷酸完美回文的一半进行了杂交。克隆于pBR322的这两个片段具有良好的模板活性，并且在体外系统中DNA复制起始于这些片段区域，在该体外系统中，如前所述，DNA复制起始于含有SV40 DNA复制起点的克隆DNA上。此外，当将这两个克隆转染到Cos细胞中时，它们能够在SV40转化的Cos细胞核中自主复制，并持续产生SV40 T抗原。这些结果表明，功能性的SV40起源样序列存在于人类基因组中，并且它们能够在产生SV40 T抗原的细胞内自主复制。
