Guangdong Province Engineering Research Center for Urban Water Recycling and Environmental Safety, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8569, Japan.
J Environ Sci (China). 2018 Nov;73:1-8. doi: 10.1016/j.jes.2017.12.020. Epub 2018 Jan 4.
Long-term exposure of nitrifiers to high concentrations of free ammonia (FA) and free nitrous acid (FNA) may affect nitrifiers activity and nitrous oxide (NO) emission. Two sequencing batch reactors (SBRs) were operated at influent ammonium nitrogen (NH-N) concentrations of 800mg/L (SBR) and 335mg/L (SBR), respectively. The NH-N removal rates in SBR and SBR were around 2.4 and 1.0g/L/day with the nitritation efficiencies of 99.3% and 95.7%, respectively. In the simulated SBR cycle, the NO emission factors were 1.61% in SBR and 2.30% in SBR. NO emission was affected slightly by FA with the emission factor of 0.22%-0.65%, while NO emission increased with increasing FNA concentrations with the emission factor of 0.22%-0.96%. The dominant ammonia oxidizing bacteria (AOB) were Nitrosomonas spp. in both reactors, and their relative proportions were 38.89% in SBR and 13.36% in SBR. Within the AOB genus, a species (i.e., operational taxonomic unit [OTU] 76) that was phylogenetically identical to Nitrosomonas europaea accounted for 99.07% and 82.04% in SBR and SBR, respectively. Additionally, OTU 215, which was related to Nitrosomonas stercoris, accounted for 16.77% of the AOB in SBR.
长期暴露于高浓度游离氨（FA）和游离亚硝酸（FNA）下可能会影响硝化菌的活性和氧化亚氮（NO）的排放。两个序批式反应器（SBR）分别以 800mg/L（SBR）和 335mg/L（SBR）的进水氨氮（NH-N）浓度运行。SBR 和 SBR 的 NH-N 去除率分别约为 2.4 和 1.0g/L/d，亚硝化效率分别为 99.3%和 95.7%。在模拟 SBR 周期中，SBR 的 NO 排放因子为 1.61%，SBR 的 NO 排放因子为 2.30%。FA 的排放因子在 0.22%-0.65%之间，对 NO 排放的影响较小，而随着 FNA 浓度的增加，NO 排放因子在 0.22%-0.96%之间增加。两个反应器中的优势氨氧化菌（AOB）均为亚硝化单胞菌属（Nitrosomonas spp.），其相对丰度分别为 SBR 中的 38.89%和 SBR 中的 13.36%。在 AOB 属内，一个与欧洲亚硝化单胞菌（Nitrosomonas europaea）系统发育完全一致的种（即 OTU 76）分别占 SBR 和 SBR 中 AOB 的 99.07%和 82.04%。此外，OTU 215 与硝化螺菌（Nitrosomonas stercoris）有关，占 SBR 中 AOB 的 16.77%。
