Ozubek Sezayi, Aktas Munir
Department of Parasitology, College of Veterinary Medicine, Firat University, 23119, Elazig, Turkey.
Department of Parasitology, College of Veterinary Medicine, Firat University, 23119, Elazig, Turkey.
Comp Immunol Microbiol Infect Dis. 2018 Aug;59:47-51. doi: 10.1016/j.cimid.2018.08.005. Epub 2018 Sep 12.
Equine piroplasmosis (EP) is a protozoon disease caused by Babesia caballi and Theileria equi transmitted by ticks from the Ixodidae family. This study investigated the genetic heterogeneity and diversity of piroplasm genotypes using the Reverse Line Blotting (RLB) technique for piroplasm species in equids in Turkey. A total of 233 blood samples from 142 horses and 91 donkeys were collected in Şanlıurfa, Tunceli, and Iğdır. The RLB assay was performed for simultaneous detection of piroplasm species. The prevalence of piroplasm infection was 33.5% (95% CI; 27.4-39.9). T. equi was the most common species (%20.2; 95% CI 15.2-25.9), whereas B. caballi infection was not detected. Nine samples hybridized to the Theileria/Babesia and Theileria catch-all probes but did not hybridize to any species-specific probe tested, suggesting the presence of unrecognized Theileria variants or genotypes. Nine samples that gave Theileria spp. signal and two samples that gave T. equi specific signal were sent for sequence analysis and the A and D genotypes of T. equi were found. In addition, T. annulata, B. ovis, and B. canis species were detected in the samples and verified by sequence analysis in Turkey. More research is needed to understand the clinical impact of atypical piroplasm infections in equids.
马焦虫病（EP）是一种由驽巴贝斯虫和马泰勒虫引起的原生动物疾病，通过硬蜱科的蜱传播。本研究使用反向线印迹（RLB）技术调查了土耳其马属动物中焦虫基因型的遗传异质性和多样性。在尚勒乌尔法、通杰利和伊兹密尔共采集了142匹马和91头驴的233份血液样本。进行RLB检测以同时检测焦虫种类。焦虫感染的患病率为33.5%（95%置信区间；27.4 - 39.9）。马泰勒虫是最常见的种类（20.2%；95%置信区间15.2 - 25.9），而未检测到驽巴贝斯虫感染。9个样本与泰勒虫/巴贝斯虫及泰勒虫通用探针杂交，但未与任何测试的种特异性探针杂交，这表明存在未识别的泰勒虫变体或基因型。9个给出泰勒虫属信号的样本和2个给出马泰勒虫特异性信号的样本被送去进行序列分析，发现了马泰勒虫的A和D基因型。此外，在样本中检测到了环形泰勒虫、绵羊巴贝斯虫和犬巴贝斯虫，并在土耳其通过序列分析得到了验证。需要更多研究来了解马属动物中非典型焦虫感染的临床影响。
