Golotin V A, Filshtein A P, Chikalovets I V, Yu Kim N, Molchanova V I, Chernikov O V
G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, Vladivostok, Russia; Far-Eastern Federal University, Vladivostok, Russia.
G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, Vladivostok, Russia.
Protein Expr Purif. 2019 Feb;154:62-65. doi: 10.1016/j.pep.2018.10.003. Epub 2018 Oct 4.
The gene of mtl from the mussel Mytilus trossulus was cloned into pET-40b(+) expression vector. After expression in E. coli using designed MX-medium an instable soluble form of MTL was obtained. The developed isolation method of the recombinant protein in "semi-denatured" conditions allowed obtaining an active soluble form of the homogenous lectin from the mussel M. trossulus (r-MTL). Both of the lectins had similar antigenic and spatial structures.
将来自紫贻贝的mtl基因克隆到pET-40b(+)表达载体中。使用设计的MX培养基在大肠杆菌中表达后，获得了不稳定的可溶性MTL形式。所开发的在“半变性”条件下重组蛋白的分离方法能够从紫贻贝中获得活性可溶性形式的同源凝集素（r-MTL）。这两种凝集素具有相似的抗原结构和空间结构。
