灵长类慢病毒基因组包装与 Gag 翻译/AUG 的关系。

Relationship between genome packaging and Gag translation/AUG of primate lentiviruses.

机构信息

Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, 565-0871, Japan.

出版信息

Microbes Infect. 2019 Mar;21(2):119-123. doi: 10.1016/j.micinf.2018.09.002. Epub 2018 Oct 4.

DOI:10.1016/j.micinf.2018.09.002

PMID:30292880

Abstract

About the relationship between retroviral genome packaging and translation, three possible modes (random-, trans-, and cis-) of packaging process could be assumed. In this report, we developed an assay system based on the RT-qPCR to measure the packaging efficiency of primate lentiviruses. With this system, we analyzed the genome packaging modes of primate lentiviruses such as HIV-1, 2, SIVmac and SIVagm. The data suggested that the modes of all viruses analyzed were very similar. In addition, we observed that the Gag-AUG sequences of them played important roles for maintaining efficient packaging, other than the initiation of translation.

摘要

关于逆转录病毒基因组包装和翻译之间的关系，可以假设包装过程的三种可能模式（随机、转位和顺式）。在本报告中，我们开发了一种基于 RT-qPCR 的测定系统来测量灵长类慢病毒的包装效率。利用该系统，我们分析了 HIV-1、2、SIVmac 和 SIVagm 等灵长类慢病毒的基因组包装模式。数据表明，分析的所有病毒的模式都非常相似。此外，我们观察到它们的 Gag-AUG 序列除了启动翻译外，对于维持有效的包装也起着重要作用。