Yang Kun, Hou Boren, Ren Fangfang, Zhou Hong, Zhao Taiqiang
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, People's Republic of China (where the research was conducted).
Department of Laboratory Medicine, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, People's Republic of China.
Biosci Biotechnol Biochem. 2019 Jan;83(1):87-94. doi: 10.1080/09168451.2018.1519386. Epub 2018 Oct 8.
Considering that fish grows in a complex aquatic environment, there is an increasing interest in fish β-defensins, which is an important group of antimicrobial peptides (AMPs). In this study, grass carp () β-defensin 1 () was isolated using homology cloning technology. Tissue distribution assay showed that transcripts were expressed with the highest levels in brain and liver, followed by some mucous tissues. To examine gcDefb1 bioactivities, the recombinant gcDefb1 proteins fused with thioredoxin tag protein (Trx) (Trx-Defb1) were induced for production in Rosetta-gami2(DE3)pLysS under optimal expression conditions. The antibacterial activity of Trx-Defb1 against was assessed and its minimum inhibitory concentration (MIC) was 36 μM. Interestingly, Trx-Defb1 significantly inhibited LPS-induced Tnf-α (gcTnf-α) secretion and nitric oxide production in grass carp head kidney monocytes/macrophages (HKM), although Trx-Defb1 alone had no effect. Our studies provide the first evidence of fish β-defensin 1 engaging in both antimicrobial and inflammation suppression process.
鉴于鱼类生长在复杂的水生环境中，人们对鱼类β-防御素越来越感兴趣，它是一类重要的抗菌肽（AMPs）。在本研究中，利用同源克隆技术分离出草鱼（）β-防御素1（）。组织分布分析表明，转录本在脑和肝脏中表达水平最高，其次是一些黏液组织。为了检测gcDefb1的生物活性，在最佳表达条件下，诱导融合硫氧还蛋白标签蛋白（Trx）（Trx-Defb1）的重组gcDefb1蛋白在Rosetta-gami2(DE3)pLysS中产生。评估了Trx-Defb1对的抗菌活性，其最小抑菌浓度（MIC）为36 μM。有趣的是，Trx-Defb1显著抑制草鱼头肾单核细胞/巨噬细胞（HKM）中LPS诱导的Tnf-α（gcTnf-α）分泌和一氧化氮产生，尽管单独的Trx-Defb1没有作用。我们的研究首次证明了鱼类β-防御素1参与抗菌和炎症抑制过程。
