Li Xiao-Juan, He Long
Department of Hematology, Jinan Third People's Hospital, Jinan 250132, Shandong Province, China.E-mail:
Department of Hematology, Jinan Third People's Hospital, Jinan 250132, Shandong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2018 Oct;26(5):1372-1377. doi: 10.7534/j.issn.1009-2137.2018.05.019.
To investigate the effect of the expression of colony-stimulating factor 1 receptor (CSF-1R), forkhead box protein 3 (FOXP3) and programmed death 1 (PD-1) protein on the prognosis of patients with Hodgkin's lymphoma.
The clinical features and treatment schemes of 54 patients with Hodgkin's lymphoma treated in our hospital were recorded, the specimens were collected and the relevant micro-environment prognostic factors such as the protein expression of CSF-1R, FOXP3 and PD-1 were detected by immunohistochemical staining, and EB virus and EB virus-encoded RNA (EBER) were detected by in situ hybridization; the correlation between CSF-1R, FOXP3, PD-1 protein expression with prognosis of patients with Hodgkin lymphoma were analyzed; both the unvariate analysis and multivariate analysis (using Cox proportional hazard model) were uased to analyze the influence factors of prognosis.
Among the 54 cases of Hodgkin's lymphoma, 22 cases (40.74%) were positive for CSF-1R, 28 cases (51.85%) showed high expression of FOXP3 and 9 cases (16.67%) were positive for PD-1. Unvariate analysis revealed that the international prognostic index (IPI) score, EBER and FOXP3 protein expression were the effecting factors of progression-free survival (PFS) of patients with Hodgkin's lymphoma (all P<0.05); clinical staging (Ann Arbor stage), IPI score, EBER, CSF-1R and FOXP3 protein expression were the effecting factors of overall survival (OS) (all P<0.05). Multivariate analysis (Cox proportional hazard model) results showed that the EBER state was the effecting factors of PFS and OS in patients with Hodgkin's lymphoma (P<0.05); the expression of FOXP3 protein was the effecting factors of PFS (P<0.05); Ann Arbor stage and the expression of CSF-1R protein were the effecting factors for OS (both P<0.05).
CSF-1R and FOXP3 closely relate with the prognosis of the patients with Hodgkin's lymphoma, which can provide a basis for targeted therapy of this disease.
探讨集落刺激因子1受体（CSF-1R）、叉头框蛋白3（FOXP3）及程序性死亡蛋白1（PD-1）的表达对霍奇金淋巴瘤患者预后的影响。
记录我院收治的54例霍奇金淋巴瘤患者的临床特征及治疗方案，采集标本，采用免疫组织化学染色法检测CSF-1R、FOXP3及PD-1蛋白等相关微环境预后因素，采用原位杂交法检测EB病毒及EB病毒编码的RNA（EBER）；分析CSF-1R、FOXP3、PD-1蛋白表达与霍奇金淋巴瘤患者预后的相关性；采用单因素分析及多因素分析（使用Cox比例风险模型）分析预后的影响因素。
54例霍奇金淋巴瘤患者中，CSF-1R阳性22例（40.74%），FOXP3高表达28例（51.85%），PD-1阳性9例（16.67%）。单因素分析显示，国际预后指数（IPI）评分、EBER及FOXP3蛋白表达是霍奇金淋巴瘤患者无进展生存期（PFS）的影响因素（均P<0.05）；临床分期（Ann Arbor分期）、IPI评分、EBER、CSF-1R及FOXP蛋白表达是总生存期（OS）的影响因素（均P<0.05）。多因素分析（Cox比例风险模型）结果显示，EBER状态是霍奇金淋巴瘤患者PFS和OS的影响因素（P<0.05）；FOXP3蛋白表达是PFS的影响因素（P<0.）；Ann Arbor分期及CSF-1R蛋白表达是OS的影响因素（均P<0.05）。
CSF-1R和FOXP3与霍奇金淋巴瘤患者的预后密切相关，可为该病的靶向治疗提供依据。
