Yu Feng, Yang Xiao-Yang, Wan Meng-Jie, Lei Mei-Qing, Sun Ling-Feng, Han Xu, Li Wen-Jin, Chen Fang-Ping
Department of Hematology, Affiliated Haikou Hospital, Xiangya School of Medicine,Central South University & Haikou Peaple's Hospital, Haikou 570208, Hainan Province, China.
Department of Hematology, Affiliated Haikou Hospital, Xiangya School of Medicine,Central South University & Haikou Peaple's Hospital, Haikou 570208, Hainan Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2018 Oct;26(5):1477-1483. doi: 10.7534/j.issn.1009-2137.2018.05.037.
To investigate the effects of synthetic long-chain polyphosphate on blood coagulation and platelet aggregation.
The effect of artificial synthetic long chain poly phosphate on blood coagulation and platelet aggregation was detected by coagulation tests, coagulation factor activity detection and platelet aggregation test, and its mechanism was explored by ELISA, flow cytometry and high content imaging system.
The long chain polyphosphates prolonged activated partial thromboplastin time, decreased coagulation factor FⅧ, FⅨ, FⅪ and FⅫ activity, blocked ADP-induced platelet aggregation, and decreased the concentration of calcium and TXA2 in platelet.
The synthetic long-chain polyphosphate can inhibit endogenous coagulation and inhibit platelet aggregation, which may be related with the inhibition of intracellular calcium and TXA2.
研究合成的长链多聚磷酸盐对血液凝固和血小板聚集的影响。
通过凝血试验、凝血因子活性检测和血小板聚集试验检测人工合成的长链多聚磷酸盐对血液凝固和血小板聚集的影响，并通过酶联免疫吸附测定法（ELISA）、流式细胞术和高内涵成像系统探索其作用机制。
长链多聚磷酸盐延长活化部分凝血活酶时间，降低凝血因子FⅧ、FⅨ、FⅪ和FⅫ的活性，阻断二磷酸腺苷（ADP）诱导的血小板聚集，并降低血小板中钙和血栓素A2（TXA2）的浓度。
合成的长链多聚磷酸盐可抑制内源性凝血并抑制血小板聚集，这可能与抑制细胞内钙和TXA2有关。
