Li Xu, Jiang Jun, Xia Jiyi, Jiang Rui
Department of Urology, Affiliated Hospital, Southwest medical University, Luzhou, China.
Department of thyroid Surgery, Affiliated Hospital, Southwest medical University, Luzhou, China.
Andrologia. 2019 Feb;51(1):e13167. doi: 10.1111/and.13167. Epub 2018 Oct 8.
The aim of this study was to investigate the relationship between sulphur dioxide (SO ) signalling pathway and the changes in erectile function under low androgen levels. Thirty-six healthy male Sprague Dawley (SD) rats aged eight weeks were randomly divided into androgen replacement group, castration group and sham group. Rats in the androgen replacement group were subcutaneously injected with testosterone propionate at 3 mg/kg every other day postcastration. The maximum intracavernous pressure/mean arterial pressure (ICP /MAP) and the relative content of SO in the penile corpus cavernosum were measured. The mRNA and protein expressions of aspartate aminotransferase (AAT1 and AAT2), cysteine oxidase (CDO), endothelial nitric oxide synthase (eNOS) and phosphorylation of endothelial nitric oxide synthase (P-eNOS) were detected. ICP /MAP, P-eNOS/eNOS and the level of SO decreased significantly in the castration group compared to the other groups (p < 0.05). The expressions of mRNA and protein decreased significantly in the castration group compared to the androgen replacement group and the sham group (p < 0.05), while there was no significant difference between the androgen replacement group and sham group. Low androgen levels can inhibit erectile function by downregulating the SO signalling pathway.
本研究旨在探讨二氧化硫（SO）信号通路与低雄激素水平下勃起功能变化之间的关系。将36只8周龄健康雄性斯普拉格-道利（SD）大鼠随机分为雄激素替代组、去势组和假手术组。雄激素替代组大鼠在去势后每隔一天皮下注射3mg/kg丙酸睾酮。测量阴茎海绵体内最大海绵体压力/平均动脉压（ICP/MAP）以及SO的相对含量。检测天冬氨酸转氨酶（AAT1和AAT2）、半胱氨酸氧化酶（CDO）、内皮型一氧化氮合酶（eNOS）的mRNA和蛋白表达以及内皮型一氧化氮合酶的磷酸化（P-eNOS）。与其他组相比，去势组的ICP/MAP、P-eNOS/eNOS和SO水平显著降低（p<0.05）。与雄激素替代组和假手术组相比，去势组的mRNA和蛋白表达显著降低（p<0.05），而雄激素替代组和假手术组之间无显著差异。低雄激素水平可通过下调SO信号通路抑制勃起功能。
