Voegel Clarissa Daniela, La Marca-Ghaemmaghami Pearl, Ehlert Ulrike, Baumgartner Markus R, Kraemer Thomas, Binz Tina Maria
Center for Forensic Hair Analytics, Zurich Institute of Forensic Medicine, University of Zurich, Zurich, Switzerland.
Clinical Psychology and Psychotherapy, Institute of Psychology, University of Zurich, Zurich, Switzerland.
Steroids. 2018 Dec;140:144-150. doi: 10.1016/j.steroids.2018.09.015. Epub 2018 Oct 5.
The retrospective analysis of endogenous steroid hormones in nails can be used to elucidate endocrine diseases and thus help with their diagnosis and treatment. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) based method was developed for the simultaneous identification and quantification of 12 steroid hormones (aldosterone, cortisone, cortisol, corticosterone, 11-deoxycortisol, androstenedione, 11-deoxycorticosterone, testosterone, dehydroepiandrosterone (DHEA), 17α-hydroxyprogesterone (17-OHP), dihydrotestosterone (DHT) and progesterone) in human fingernails. Steroid hormones were extracted from 0.5 mg to 10 mg pulverized nail clippings by methanolic extraction, followed by a liquid-liquid extraction. The analysis was conducted with LC-MS/MS in electrospray ionization positive mode. The method was validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, matrix effect, recovery and robustness. It was successfully applied for steroid profiling in nails of mothers and their infants where cortisol, cortisone, testosterone, progesterone, androstenedione and 11-deoxycorticosterone could be detected. Furthermore, it could be shown that there is no significant difference in concentrations between left and right hand for cortisol, cortisone and progesterone. A positive linear correlation between cortisol and cortisone in nails was found. In conclusion, it could be shown that nails are a suitable matrix for the retrospective monitoring of cumulative steroid hormone levels.
对指甲中内源性甾体激素进行回顾性分析，可用于阐明内分泌疾病，从而有助于其诊断和治疗。开发了一种基于液相色谱-串联质谱（LC-MS/MS）的方法，用于同时鉴定和定量人指甲中的12种甾体激素（醛固酮、可的松、皮质醇、皮质酮、11-脱氧皮质醇、雄烯二酮、11-脱氧皮质酮、睾酮、脱氢表雄酮（DHEA）、17α-羟孕酮（17-OHP）、双氢睾酮（DHT）和孕酮）。通过甲醇提取，然后进行液-液萃取，从0.5mg至10mg的粉碎指甲剪屑中提取甾体激素。采用LC-MS/MS在电喷雾电离正模式下进行分析。该方法在线性、检测限（LOD）、定量限（LOQ）、精密度、准确度、基质效应、回收率和稳健性方面得到了验证。该方法成功应用于母亲及其婴儿指甲中的甾体激素谱分析，可检测到皮质醇、可的松、睾酮、孕酮、雄烯二酮和11-脱氧皮质酮。此外，还表明左手和右手的皮质醇、可的松和孕酮浓度无显著差异。发现指甲中皮质醇和可的松之间呈正线性相关。总之，可以证明指甲是回顾性监测累积甾体激素水平的合适基质。
