Université de Brest, EA3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, UMT14.01 SPORE-RISK, 6 Rue de L'Université, F-29334, Quimper, France.
ADRIA Développement, UMT14.01 SPORE-RISK, ZA Creac'h Gwen, F-29196, Quimper, France.
Food Microbiol. 2019 Feb;77:21-25. doi: 10.1016/j.fm.2018.08.005. Epub 2018 Aug 14.
Predicting the microbial safety of food products stored in modified atmosphere packaging implies taking into account the effect of oxygen reduction on microbial growth. According to their respiratory-type, the micro-organisms are not impacted similarly by the oxygen concentration. The aim of this article was to quantify and model the oxygen effect on the growth rates of 5 bacterial species: Listeria monocytogenes and Bacillus weihenstephanensis (facultative anaerobic), Pseudomonas fluorescens (strict aerobic), Clostridium perfringens and Clostridium sporogenes (strict anaerobic). The results showed the oxygen concentration doesn't modify the behavior of both facultative anaerobic strains. The growth rate of P. fluorescens decreased with the oxygen concentration, but the effect is only noticeable when the oxygen concentration fell below 3% in the gaseous phase. Conversely, the oxygen acted as a growth inhibitor for both Clostridium species. But total inhibition is reached only for 3.26% and 6.61% respectively for C. sporogenes and C. perfringens. Two models have been fitted for both respiratory-types, the first is the Monod model considering oxygen as a substrate for growth, and the second is the classic inhibitory model based on minimal inhibitory concentration.
预测贮藏于气调包装中的食品的微生物安全性，需要考虑到氧气减少对微生物生长的影响。根据其呼吸类型，微生物对氧气浓度的影响并不相同。本文的目的是量化和模拟氧气对 5 种细菌的生长速率的影响：单核细胞增生李斯特菌和魏氏芽孢杆菌（兼性厌氧菌）、荧光假单胞菌（严格需氧菌）、产气荚膜梭菌和脆弱拟杆菌（严格厌氧菌）。结果表明，氧气浓度并不改变两种兼性厌氧菌的行为。荧光假单胞菌的生长速率随着氧气浓度的降低而降低，但只有当气相中的氧气浓度下降到 3%以下时，这种影响才会变得明显。相反，氧气对两种梭菌都起到了生长抑制剂的作用。但是，对于脆弱拟杆菌和产气荚膜梭菌，分别只有当氧气浓度达到 3.26%和 6.61%时才会完全抑制。对于这两种呼吸类型，已经拟合了两种模型，第一种是考虑氧气作为生长基质的 Monod 模型，第二种是基于最小抑菌浓度的经典抑制模型。
