Department of Chemical Engineering, Monash University, Clayton, Victoria, 3800, Australia.
Biotechnol J. 2019 Apr;14(4):e1800487. doi: 10.1002/biot.201800487. Epub 2018 Oct 18.
The unique strained ring structure in cyclopropane fatty acids (CFA) conveys oxidative stability and lubricity to lipids. These attributes are highly valuable for industrial applications such as cosmetics and specialist lubrication but there is currently no commercial source of the lipid. Here, built on recently engineered strains of Saccharomyces cerevisiae, the authors have developed an efficient strategy for CFA production. Expression of the Escherichia coli cyclopropane fatty acid synthetase (Ec.CFAS) in the engineered yeast resulted in formation of cis-9,10-methylene-hexadecanoic and octadecanoic acids in both the phospholipid (PL) and triacylglycerol (TAG) fractions. CFA concentration in TAG of engineered yeast is 12 mg CFA g DCW (fourfold above the strain expressing CFAS only). The yield of CFA increases from 13.2 to 68.3 mg L , the highest reported in yeast, using a two-stage bioprocess strategy that separated cell growth from the lipid modification stage. Strategies for further improvement of this valuable lipid are proposed.
环丙烷脂肪酸 (CFA) 的独特张力环结构赋予了脂质氧化稳定性和润滑性。这些特性对化妆品和特种润滑剂等工业应用非常有价值，但目前没有商业来源的脂质。在最近工程化的酿酒酵母菌株的基础上，作者开发了一种有效的 CFA 生产策略。在工程酵母中表达大肠杆菌环丙烷脂肪酸合成酶 (Ec.CFAS) 导致顺式-9,10-亚甲基-十六烷酸和十八烷酸在磷脂 (PL) 和三酰基甘油 (TAG) 两部分中形成。工程酵母中 TAG 中的 CFA 浓度为 12mg CFA g DCW（仅表达 CFAS 的菌株的四倍）。使用将细胞生长与脂质修饰阶段分开的两段式生物过程策略，CFA 的产量从 13.2 增加到 68.3mg L，是酵母中报道的最高产量。提出了进一步提高这种有价值脂质的策略。
